Shandong Peanut Research Institute, Qingdao, Shandong, China.
Tobacco Research Institute of CAAS, Qingdao, Shandong, China.
Proteomics. 2021 Mar;21(5):e2000156. doi: 10.1002/pmic.202000156. Epub 2021 Feb 23.
Lysine crotonylation is an important post-translational modification process. Most research in this area has been carried out on mammals and yeast, but there has been little research on it in plants. In the current study, large-scale lysine crotonylome analysis was performed by a combination of affinity enrichment and high-resolution LC-MS/MS analysis. Altogether, 6051 lysine crotonylation sites were identified in 2508 protein groups. Bioinformatics analysis showed that lysine-crotonylated proteins were involved in many biological processes, such as carbon fixation in photosynthetic organisms, biosynthesis of amino acids, ribosomes structure and function. In particular, subcellular localization analysis showed that 43% of the crotonylated proteins were located in the chloroplast. Twenty-nine crotonylation proteins were associated with photosynthesis and functional enrichment that these proteins were associated with the reaction center, photosynthetic electron transport, and ATP synthesis. Based on these results, further studies to expand on the lysine crotonylome analysis were suggested.
赖氨酸丁酰化是一种重要的翻译后修饰过程。该领域的大多数研究都集中在哺乳动物和酵母上，但在植物中研究甚少。在本研究中，通过亲和富集和高分辨率 LC-MS/MS 分析的组合进行了大规模的赖氨酸丁酰化组分析。总共在 2508 个蛋白质组中鉴定出了 6051 个赖氨酸丁酰化位点。生物信息学分析表明，赖氨酸丁酰化蛋白参与了许多生物过程，如光合生物中的碳固定、氨基酸的生物合成、核糖体的结构和功能。特别是，亚细胞定位分析表明，43%的丁酰化蛋白位于叶绿体中。29 个丁酰化蛋白与光合作用有关，功能富集表明这些蛋白与反应中心、光合电子传递和 ATP 合成有关。基于这些结果，建议进一步研究赖氨酸丁酰化组分析。
