Laboratory of Biochemistry and Biomedical Materials, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, PR China.
Laboratory of Biochemistry and Biomedical Materials, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, PR China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, PR China.
Carbohydr Polym. 2021 Mar 15;256:117519. doi: 10.1016/j.carbpol.2020.117519. Epub 2020 Dec 17.
Vitreous, an essential dioptric medium for the human eyes, must be filled with artificial materials once damaged. Carboxymethyl chitosan (CMCTS) is one of the most important water-soluble chitosan derivatives with improved biocompatibility and biodegradability. In this study, oxidized hyaluronic acid (OHA) was prepared as crosslinking reagent. CMCTS and OHA were used to develop a biocompatible, self-repairing and in-situ injectable hydrogel for vitreous substitutes. Results showed the hydrogel with controllable swelling properties, high transparency, acceptable cytocompatibility on mouse fibroblast L929 and histocompatibility in vivo. Furthermore, hydrogel was injected in-situ into the vitreous cavity after vitrectomy on New Zealand Rabbits, no significant and persistent adverse effects were observed during the 90-day follow-up period. In addition, the hydrogel maintained intraocular pressure of the operated eyes and the inherent position of the retina. Collectively, this injectable, biodegradable, nontoxic hydrogel possessed enormous potential to become a vitreous substitute material.
玻璃体是人类眼睛重要的屈光介质，一旦受损必须用人工材料填充。羧甲基壳聚糖（CMCTS）是最重要的水溶性壳聚糖衍生物之一，具有改善的生物相容性和生物降解性。在本研究中，氧化透明质酸（OHA）被制备为交联试剂。CMCTS 和 OHA 用于开发一种用于玻璃体替代物的具有生物相容性、自修复和可原位注射的水凝胶。结果表明，该水凝胶具有可控制的溶胀性能、高透明度、对小鼠成纤维细胞 L929 的可接受细胞相容性和体内组织相容性。此外，在新西兰兔玻璃体切除术后将水凝胶原位注入玻璃体腔，在 90 天的随访期间未观察到明显和持续的不良反应。此外，水凝胶保持了手术眼的眼内压和视网膜的固有位置。总的来说，这种可注射、可生物降解、无毒的水凝胶具有成为玻璃体替代材料的巨大潜力。
