College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong, China.
School of Medicine, Heze Medical College, Heze, Shandong, China.
J Biomed Mater Res A. 2018 Jul;106(7):1997-2006. doi: 10.1002/jbm.a.36403. Epub 2018 Apr 16.
Although various biopolymers and synthetic compounds have been proposed and tested as the vitreous substitutes, no ideal material has been identified yet. In the present study, we developed an in situ-formed hydrogel by crosslinking hydroxypropyl chitosan with alginate dialdehyde. Physical properties of the hydrogel were studied and the cytotoxicity was evaluated using L929 fibroblasts, rabbit corneal endothelial cells and retinal pigment epithelial cells. In a preliminary in vivo study, the hydrogel was employed as vitreous substitute after vitrectomy surgery on rabbits and multiple parameters indicating biosafety and biocompatibility were measured and analyzed postoperatively. Our results showed that the refractive index, transmittance, pH value and density of the hydrogel were similar to those of human vitreous. Cytotoxicity tests demonstrated the hydrogel to be nontoxic to all of the three cell lines selected. Using the rabbit model, we showed that the hydrogel could form in situ and postoperative analysis of slit-lamp observation, intraocular pressure, corneal endothelium examination, B-scan ultrasound and fundus photography showed no significant adverse reactions in the operated eyes during the 90-day follow-up. However, electroretinogram and histopathological examinations indicated minor vision decline and decrease of the densities of cones and rods in the operated rabbit eyes. Collectively, our study suggested that the in situ-formed hydrogel could potentially be used as a vitreous substitute, with its long-term safety and efficacy to be further assessed. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1997-2006, 2018.
尽管已经提出并测试了各种生物聚合物和合成化合物作为玻璃体替代物，但尚未确定理想的材料。在本研究中，我们通过将羟丙基壳聚糖与海藻酸钠二醛交联来开发原位形成的水凝胶。研究了水凝胶的物理性质，并使用 L929 成纤维细胞、兔角膜内皮细胞和视网膜色素上皮细胞评估了细胞毒性。在初步的体内研究中，该水凝胶在兔眼玻璃体切除术后用作玻璃体替代物，并在术后测量和分析了多个表明生物安全性和生物相容性的参数。我们的结果表明，水凝胶的折射率、透光率、pH 值和密度与人玻璃体相似。细胞毒性试验表明，该水凝胶对所选的三种细胞系均无毒性。使用兔模型，我们表明水凝胶可以原位形成，术后裂隙灯观察、眼压、角膜内皮检查、B 型超声和眼底照相分析显示，在 90 天的随访期间，手术眼无明显不良反应。然而，视网膜电图和组织病理学检查表明，手术兔眼的视力略有下降，视锥和视杆的密度降低。总的来说，我们的研究表明，原位形成的水凝胶可能可用作玻璃体替代物，其长期安全性和有效性有待进一步评估。© 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1997-2006, 2018.
