Department of Mechanical Engineering and Materials Science, Institute of Materials Science and Engineering, Washington University in St Louis, St Louis, MO, USA.
Department of Medicine, Division of Bone and Mineral Diseases, Washington University in St Louis, St Louis, MO, USA.
Nat Biomed Eng. 2021 Jan;5(1):64-76. doi: 10.1038/s41551-020-00672-y. Epub 2021 Jan 22.
The detection and quantification of protein biomarkers in interstitial fluid is hampered by challenges in its sampling and analysis. Here we report the use of a microneedle patch for fast in vivo sampling and on-needle quantification of target protein biomarkers in interstitial fluid. We used plasmonic fluor-an ultrabright fluorescent label-to improve the limit of detection of various interstitial fluid protein biomarkers by nearly 800-fold compared with conventional fluorophores, and a magnetic backing layer to implement conventional immunoassay procedures on the patch and thus improve measurement consistency. We used the microneedle patch in mice for minimally invasive evaluation of the efficiency of a cocaine vaccine, for longitudinal monitoring of the levels of inflammatory biomarkers, and for efficient sampling of the calvarial periosteum-a challenging site for biomarker detection-and the quantification of its levels of the matricellular protein periostin, which cannot be accurately inferred from blood or other systemic biofluids. Microneedle patches for the minimally invasive collection and analysis of biomarkers in interstitial fluid might facilitate point-of-care diagnostics and longitudinal monitoring.
在间质液中检测和定量蛋白质生物标志物受到采样和分析方面的挑战的阻碍。在这里，我们报告了使用微针贴片进行快速体内采样和在针上对间质液中的目标蛋白质生物标志物进行定量分析。我们使用等离子体荧光素-一种超亮荧光标记物-将各种间质液蛋白质生物标志物的检测限提高了近 800 倍，与传统荧光团相比，并使用磁性背衬层在贴片上实施常规免疫测定程序，从而提高了测量的一致性。我们在小鼠中使用微针贴片进行微创评估可卡因疫苗的效率，进行炎症生物标志物水平的纵向监测，并有效地采样颅盖骨骨膜-这是生物标志物检测的一个具有挑战性的部位-以及对基质细胞蛋白骨膜蛋白水平的定量分析，从血液或其他全身生物流体中无法准确推断其水平。用于微创采集和分析间质液中生物标志物的微针贴片可能有助于即时诊断和纵向监测。
