Federal University of Campina Grande, Sítio Olho d'água da Bica, 58175-000, Cuité, Paraíba State, Brazil; Laboratory of Experimental Nutrition, Department of Nutrition, Federal University of Campina Grande, Sítio Olho d'água da Bica, 58175-000, Cuité, Paraíba State, Brazil.
Laboratory of Experimental Nutrition, Department of Nutrition, Federal University of Campina Grande, Sítio Olho d'água da Bica, 58175-000, Cuité, Paraíba State, Brazil; Federal University of Paraiba, University City, 58051-900, João Pessoa, Paraíba State, Brazil.
J Ethnopharmacol. 2021 Apr 24;270:113862. doi: 10.1016/j.jep.2021.113862. Epub 2021 Jan 21.
Phyllanthus niruri L. (Phyllanthaceae) is a plant used in traditional medicine, mainly to treat kidney stones. However, the effects of maternal exposure to P. niruri remain poorly explored.
The objective of this study was to investigate the effects of administration of aqueous extract of P. niruri (AEPN) during pregnancy and lactation, in maternal toxicity, reflex maturation, and offspring memory.
Pregnant rats were divided into three groups (n = 8/group): Control (vehicle), AEPN 75, and AEPN 150 (each respectively treated with P. niruri at a dose of 75 and 150 mg/kg/day). The animals were treated via intragastric gavage during pregnancy and lactation. Weight gain, feed intake, and reproductive performance were analyzed in the mothers. In the offspring, the following tests were performed: Neonatal Reflex Ontogeny, Open Field Habituation Test and the Object Recognition Test in adulthood.
Maternal exposure to AEPN did not influence weight gain, feed intake, or reproductive parameters. In the offspring, anticipation of reflex ontogenesis (time of completion) was observed (p < 0.05). During adulthood, the AEPN groups presented decreases in exploratory activity upon their second exposure to the Open Field Habituation Test (in a dose-dependent manner) (p < 0.05). In the Object Recognition Test, administration of the extract at 75 and 150 mg/kg induced significant dose-dependent improvements in short and long-term memory (p < 0.05).
Administration of the AEPN accelerated the reflex maturation in neonates, and improved offspring memory while inducing no maternal or neonatal toxicity.
叶下珠（叶下珠科）是一种在传统医学中被用于治疗肾结石的植物。然而，母体暴露于叶下珠的影响仍未得到充分探索。
本研究旨在探讨妊娠和哺乳期给予叶下珠水提物（AEPN）对母体毒性、反射成熟度和后代记忆的影响。
将怀孕的大鼠分为三组（每组 8 只）：对照组（载体）、AEPN75 和 AEPN150（分别用 75 和 150mg/kg/天的叶下珠处理）。动物在妊娠和哺乳期通过灌胃进行治疗。分析母亲的体重增加、饲料摄入量和生殖性能。在后代中，进行以下测试：新生儿反射发育、旷场习惯化测试和成年期物体识别测试。
母体暴露于 AEPN 不影响体重增加、饲料摄入量或生殖参数。在后代中，观察到反射发育的预期（完成时间）提前（p<0.05）。在成年期，AEPN 组在第二次暴露于旷场习惯化测试时表现出探索活动的减少（呈剂量依赖性）（p<0.05）。在物体识别测试中，以 75 和 150mg/kg 给予提取物可显著改善短期和长期记忆（呈剂量依赖性）（p<0.05）。
AEPN 的给予加速了新生儿的反射成熟，并改善了后代的记忆，同时没有引起母体或新生儿毒性。
