Kumthip Kattareeya, Khamrin Pattara, Ushijima Hiroshi, Maneekarn Niwat
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand.
Appl Environ Microbiol. 2021 Jul 27;87(16):e0045621. doi: 10.1128/AEM.00456-21.
Human bocavirus (HBoV) has been recognized as an important pathogen that causes respiratory infection and acute gastroenteritis in young children worldwide. HBoV is most likely transmitted by the respiratory route and by fecal-oral transmission. Recently, HBoV has been detected in several types of environmental water and in bivalve shellfish. However, study of the existence of HBoV in oysters is still undocumented in Thailand. In this study, 144 oyster samples collected from different markets in Chiang Mai, Thailand, in 2017 and 2018 were investigated for the presence of HBoV by nested PCR and sequencing. HBoV was detected in 11 out of 144 samples (7.6%). Nine HBoV-positive samples (81.8%) were identified as genotype 1 (HBoV1) and two (18.2%) as HBoV2. A monthly investigation of HBoV in oyster samples from July 2017 to June 2018 showed that HBoV was sporadically detected in particular months spanning the rainy and colder season, with a peak in January. This study demonstrates the presence and genotype diversity of HBoV in oyster samples in Thailand. The findings contribute to evaluating the risk of foodborne transmission of HBoV and to monitoring outbreaks of HBoV in Thailand and in other countries. Human bocavirus is recognized as an important cause of respiratory infection and of acute gastroenteritis in children worldwide. Human bocavirus has been widely detected in many clinical specimens, as well as in several types of environmental samples. Most previous studies describe the incidence of bocavirus infection in humans, whereas few data are available for the occurrence of human bocavirus in food materials, particularly that in bivalve shellfish. Our findings provide evidence for the existence and prevalence of human bocavirus in oysters, suggesting that further monitoring of the potential risk of food- and waterborne transmission of this virus to humans should be undertaken.
人博卡病毒（HBoV）已被公认为一种重要病原体，可在全球范围内导致幼儿呼吸道感染和急性胃肠炎。HBoV很可能通过呼吸道途径以及粪口传播。最近，在几种环境水样和双壳贝类中检测到了HBoV。然而，泰国尚未有关于牡蛎中HBoV存在情况的研究记录。在本研究中，对2017年和2018年从泰国清迈不同市场采集的144份牡蛎样本进行了巢式PCR和测序，以检测HBoV的存在情况。144份样本中有11份（7.6%）检测到HBoV。9份HBoV阳性样本（81.8%）被鉴定为1型（HBoV1），2份（18.2%）为HBoV2。对2017年7月至2018年6月牡蛎样本进行的每月HBoV调查显示，在雨季和较冷季节的特定月份偶尔检测到HBoV，1月份出现峰值。本研究证明了泰国牡蛎样本中HBoV的存在及其基因型多样性。这些发现有助于评估HBoV经食物传播的风险，并监测泰国及其他国家的HBoV疫情。人博卡病毒被认为是全球儿童呼吸道感染和急性胃肠炎的重要病因。人博卡病毒已在许多临床标本以及几种环境样本中广泛检测到。此前大多数研究描述了博卡病毒在人类中的感染发生率，而关于人博卡病毒在食品材料尤其是双壳贝类中的存在情况的数据很少。我们的研究结果为牡蛎中存在人博卡病毒及其流行情况提供了证据，表明应进一步监测该病毒经食物和水传播给人类的潜在风险。
