AkyÜz Esin, TÜrkoĞlu Sercan, SÖzgen BaŞkan Kevser, TÜtem Esma, Apak Mustafa Reşat
Department of Chemistry, Faculty of Engineering, İstanbul University-Cerrahpaşa, İstanbul Turkey.
Sem Laboratory Equipments Marketing Industry and Trade Inc., İstanbul Turkey.
Turk J Chem. 2020 Dec 16;44(6):1663-1673. doi: 10.3906/kim-2007-67. eCollection 2020.
In this study, the total phenolic contents and total antioxidant capacities of some commercial bitter melon products (powder, packaged powder, capsule, paste in olive oil), and of unripe and ripe fruits were determined by spectrophotometric and chromatographic methods. The total antioxidant capacities of unripe and ripe bitter melon samples, determined by using the CUPRAC (cupric reducing antioxidant capacity assay) and ABTS (2,2'-azino-bis(3-ethylbenzthiazolin-6-sulfonic acid))/HRP (horseradish peroxidase) methods, were 42.5 and 36.3 µmol TRE (Trolox equivalent) g-1, and 8.7 and 7.0 µmol TRE g-1, respectively. The TAC (total antioxidant capacity) order of the studied samples using the same 2 methods were determined as follows: capsule (CUPRAC value, 140.8; ABTS/HRP value, 143.6 µmol TRE g-1) > packaged powder (129.6; 126.1) > powder (52.3; 64.3) > unripe fruit (42.5; 36.3) > paste in olive oil (17.6; 14.4) > ripe fruit (8.7; 7.0). The order of phenolic content was found as follows: unripe fruit (193.2 µmol GAE (gallic acid equivalent) g-1) > capsule (162.0) > packaged powder (160.6) > powder (83.6) > paste in olive oil (38.3) > ripe fruit (14.6).
在本研究中,采用分光光度法和色谱法测定了一些市售苦瓜产品(粉末、包装粉末、胶囊、橄榄油糊剂)以及未成熟和成熟果实的总酚含量和总抗氧化能力。使用CUPRAC(铜离子还原抗氧化能力测定法)和ABTS(2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸))/HRP(辣根过氧化物酶)方法测定的未成熟和成熟苦瓜样品的总抗氧化能力分别为42.5和36.3 μmol TRE(Trolox当量)g-1,以及8.7和7.0 μmol TRE g-1。使用相同的两种方法测定的研究样品的TAC(总抗氧化能力)顺序如下:胶囊(CUPRAC值,140.8;ABTS/HRP值,143.6 μmol TRE g-1)>包装粉末(129.6;126.1)>粉末(52.3;64.3)>未成熟果实(42.5;36.3)>橄榄油糊剂(17.6;14.4)>成熟果实(8.7;7.0)。酚含量顺序如下:未成熟果实(193.2 μmol GAE(没食子酸当量)g-1)>胶囊(162.0)>包装粉末(160.6)>粉末(83.6)>橄榄油糊剂(38.3)>成熟果实(14.6)。