Shukla Pushp Sheel, Prithiviraj Balakrishnan
Marine Bio-Products Research Laboratory, Department of Plant, Food and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS, Canada.
Front Plant Sci. 2021 Jan 8;11:601843. doi: 10.3389/fpls.2020.601843. eCollection 2020.
Phosphorous is one of the major limiting factors determining plant growth. Current agricultural practices mainly rely on the use of chemical fertilizers posing threat to the ecosystem. In this study, the application of an extract (ANE) in phosphorous (P)-limited conditions improved the fresh and dry weight of shoots and roots of . ANE-treated grown under P-limited conditions showed a higher P content than the control. ANE activated simultaneous responses, at multiple levels, in grown under P-limited conditions as seen from the regulation of gene expression at the whole-plant level to specific biochemical responses on a subcellular level. ANE-supplemented grown under P-limited conditions also showed reduced electrolyte leakage and lipid peroxidation by an improved membrane stability. ANE treatment reduced P-limitation-induced oxidative damage in by reducing HO and accumulation. Furthermore, ANE also induced the accumulation of the total contents of soluble sugars, amino acids, phenolics, and flavonoids. Gene expression analysis suggested that ANE differentially modulated the expression of P-starvation responsive genes involved in metabolic, signal transduction, and developmental pathways in . ANE also modulated the expression of genes involved in sugar, lipid, and secondary metabolism. Thus, this study illustrated the role of ANE in improving the productivity of , an important crop, in P-limited conditions. Furthermore, it sets the framework to increase agricultural productivity in nutrient deficient soils using a sustainable, eco-friendly strategy.
磷是决定植物生长的主要限制因素之一。当前的农业实践主要依赖使用对生态系统构成威胁的化肥。在本研究中，在磷（P）受限条件下施用一种提取物（ANE）提高了[植物名称未给出]地上部和根部的鲜重和干重。在磷受限条件下生长的经ANE处理的[植物名称未给出]比对照显示出更高的磷含量。从全株水平的基因表达调控到亚细胞水平的特定生化反应可以看出，ANE在磷受限条件下生长的[植物名称未给出]中在多个水平上激活了同步反应。在磷受限条件下生长的补充了ANE的[植物名称未给出]还通过改善膜稳定性降低了电解质渗漏和脂质过氧化。ANE处理通过减少羟基自由基（HO）和[其他自由基未给出]的积累降低了磷受限诱导的[植物名称未给出]中的氧化损伤。此外，ANE还诱导了可溶性糖、氨基酸、酚类化合物和黄酮类化合物总含量的积累。基因表达分析表明，ANE差异调节了[植物名称未给出]中参与代谢、信号转导和发育途径的磷饥饿响应基因的表达。ANE还调节了参与糖、脂质和次生代谢的基因的表达。因此，本研究阐明了ANE在磷受限条件下提高重要作物[植物名称未给出]生产力方面的作用。此外它还建立了一个框架，以采用可持续、生态友好的策略提高养分缺乏土壤中的农业生产力。
