Che Soh Nor 'Awatif, Rapi Hannah Syahirah, Mohd Azam Nurul Shahirah, Santhanam Ramesh Kumar, Assaw Suvik, Haron Mohd Nizam, Ali Abdul Manaf, Maulidiani M, Idris Izwandy, Ismail Wan Iryani Wan
Cell Signaling and Biotechnology Research Group (CeSBTech), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
Biological Security and Sustainability (BioSeS) Research Group, Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
Evid Based Complement Alternat Med. 2020 Dec 28;2020:6688084. doi: 10.1155/2020/6688084. eCollection 2020.
which is colloquially known as Ruat Sarung can be found along the west coast of Peninsular Malaysia. The species has a unique ability to regenerate anterior and posterior segments upon self-amputation or injury, thus having potential as a wound healing promoter. In this study, the wound healing potential of aqueous extract on acute wound model in rats was revealed for the first time. Various concentrations (0.1%, 0.5%, and 1.0% w/w) of ointment were formulated and tested on Sprague Dawley rats through topical application on full-thickness skin wounds for 14 days. The wound healing effects were investigated via behaviour observation, wound contraction, and histopathological analysis. Quality assessment was performed via skin irritation test, microbial contamination test (MCT), and heavy metal detection. The study also included test for antibacterial activities and detection of bioactive compounds in . One percent of ointment showed rapid wound healing potential with good soothing effects and more collagen deposition in comparison to the commercial wound healing ointments such as acriflavine (0.1% w/v) and traditional ointment gamat (sea cucumber extract) (15.0% w/v). No local skin irritation, microbial contamination, and insignificant concentration of heavy metals were observed, which indicate its safe application. Moreover, the aqueous extract of exhibited antibacterial activities against and with minimum inhibitory concentration (MIC) value at 0.4 g/ml. H NMR analysis of the aqueous extract of revealed some metabolites that might be responsible for its wound healing properties such as amino acids, halogenated aromatics, organic acids, vitamins, and others. Altogether, these results suggested that the aqueous extract of could be utilised as an alternative natural wound healing promoter.
通俗称为Ruat Sarung的[物种名称未给出]可在马来西亚半岛西海岸找到。该物种具有在自我切断或受伤后再生前后段的独特能力，因此具有作为伤口愈合促进剂的潜力。在本研究中，首次揭示了[物种名称未给出]水提取物对大鼠急性伤口模型的伤口愈合潜力。配制了不同浓度（0.1%、0.5%和1.0% w/w）的软膏，并通过在Sprague Dawley大鼠的全层皮肤伤口上局部应用14天进行测试。通过行为观察、伤口收缩和组织病理学分析来研究伤口愈合效果。通过皮肤刺激性试验、微生物污染试验（MCT）和重金属检测进行质量评估。该研究还包括抗菌活性测试和[物种名称未给出]中生物活性化合物的检测。与吖啶黄（0.1% w/v）和传统软膏gamat（海参提取物）（15.0% w/v）等商业伤口愈合软膏相比，1%的[物种名称未给出]软膏显示出快速的伤口愈合潜力，具有良好的舒缓效果和更多的胶原蛋白沉积。未观察到局部皮肤刺激、微生物污染以及重金属浓度不显著，这表明其应用安全。此外，[物种名称未给出]的水提取物对[细菌名称未给出]和[细菌名称未给出]具有抗菌活性，最低抑菌浓度（MIC）值为0.4 g/ml。[物种名称未给出]水提取物的1H NMR分析揭示了一些可能对其伤口愈合特性负责的代谢物，如氨基酸、卤代芳烃、有机酸、维生素等。总之，这些结果表明[物种名称未给出]的水提取物可作为一种替代的天然伤口愈合促进剂。
