Ahmad Nazri Mohd Ulul Ilmie, Mahmud Mohd Hafizi, Saidi Basri, Mat Isa Mohd Noor, Ehsak Zolkapli, Ross Othman, Idris Izwandy, Ismail Wan Iryani Wan
Cell Signalling and Biotechnology Research Group (CeSBTech), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia.
Biological Security and Sustainability Research Group (BIOSES), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia.
Heliyon. 2021 Feb 25;7(2):e06307. doi: 10.1016/j.heliyon.2021.e06307. eCollection 2021 Feb.
The polychaete Grube, 1878 is among those organisms successfully carrying out full body regeneration, including the whole nervous system. Thus, potentially can be regarded for the nervous system regeneration (NSR) study. However, data on the property of its nervous system and the NSR profile are still lacking. In this study, we investigated the morphology of anterior nervous system (ANS) and examined the cellular and molecular profiles on its early anterior NSR. The nervous system of consists of a symmetry brain with nerves branching off, circumpharyngeal connectives that connect the brain and nerve cord as well as obvious segmental ganglia. Moreover, we identified changes in the cellular condition of the ganglionic cells in the regenerating tissue, such as the accumulation of lysosomes and lipofuscins, elongated mitochondria and multiple nucleoli. Furthermore, mRNA of tissues at two regenerating stages, as well as intact tissue (non-regenerating), were sequenced with Illumina sequencer. We identified from these tissues 37,248 sequences, 18 differential expressed proteins of which upregulated were involved in NSR with noelin-like isoform X2 turned up to be the highest being expressed. Our results highlight the cellular and molecular changes during early phase of NSR, thus providing essential insights on regeneration within Annelida and understanding the neurodegenerative diseases.
多毛纲动物格鲁贝，1878年被发现，是那些能够成功进行全身再生的生物之一，包括整个神经系统。因此，它有可能被用于神经系统再生（NSR）研究。然而，关于其神经系统特性和NSR概况的数据仍然缺乏。在这项研究中，我们研究了前部神经系统（ANS）的形态，并检查了其早期前部NSR的细胞和分子概况。该动物的神经系统由一个对称的脑、分支的神经、连接脑和神经索的围咽结缔组织以及明显的节段性神经节组成。此外，我们还确定了再生组织中神经节细胞的细胞状况变化，如溶酶体和脂褐素的积累、线粒体伸长和多个核仁。此外，使用Illumina测序仪对两个再生阶段的组织以及完整组织（非再生组织）的mRNA进行了测序。我们从这些组织中鉴定出37248个序列，其中18种差异表达蛋白上调，参与NSR，其中无elin样异构体X2表达量最高。我们的结果突出了NSR早期阶段的细胞和分子变化，从而为环节动物的再生以及理解神经退行性疾病提供了重要的见解。
