O'Donoghue John L, Beevers Carol, Buard Annie
Department of Environmental Medicine, School of Medicine and Dentistry, University of Rochester, Box EHSC, 601 Elmwood Ave., Rochester, NY, 14642, United States.
Exponent International Ltd, The Lenz, Hornbeam Park, Harrogate, HG2 8RE, United Kingdom.
Toxicol Rep. 2021 Jan 11;8:206-214. doi: 10.1016/j.toxrep.2021.01.005. eCollection 2021.
Hydroquinone (HQ) exposure is common as it is a natural component of plant-based foods and is used in some fingernail polishes, hair dyes, and skin lighteners. Industrially it is used as an antioxidant, polymerization inhibitor, and reducing agent. The current study was undertaken to determine whether HQ may cause DNA damage in an comet assay in F344 rats. DNA strand breaks were assessed in the duodenum as a direct tissue contact site, the testes, and the liver and kidneys, which were tumor sites in bioassays. Rats were exposed to HQ by gavage at 0, 105, 210, or 420 mg/kg/day. At all dose levels, mean % tail intensity and tail moment values for all tissues in animals given HQ were similar to the control. There were no statistically significant increases in tail intensity in any tissue following HQ treatment of male and female rat and data for all animals fell within the available historical control ranges for each tissue. There was no evidence of induction of DNA damage in cells isolated from duodenum, kidney or liver of male and female rats or in the testes of male rats following exposure to HQ at a dose levels up to 420 mg/kg/day, which caused acute renal necrosis.
对苯二酚（HQ）暴露很常见，因为它是植物性食物的天然成分，并且用于某些指甲油、染发剂和皮肤美白剂中。在工业上，它用作抗氧化剂、聚合抑制剂和还原剂。当前的研究旨在通过彗星试验确定HQ是否会在F344大鼠中导致DNA损伤。在十二指肠（作为直接的组织接触部位）、睾丸以及肝脏和肾脏（在生物测定中为肿瘤发生部位）中评估DNA链断裂情况。大鼠通过灌胃接受0、105、210或420mg/kg/天的HQ暴露。在所有剂量水平下，给予HQ的动物所有组织的平均尾强度百分比和尾矩值与对照组相似。对雄性和雌性大鼠进行HQ处理后，任何组织中的尾强度均无统计学上的显著增加，并且所有动物的数据均落在每个组织可用的历史对照范围内。在暴露于高达420mg/kg/天的HQ后，在雄性和雌性大鼠的十二指肠、肾脏或肝脏分离的细胞中以及雄性大鼠的睾丸中，均没有诱导DNA损伤的证据，该剂量导致了急性肾坏死。
