Wu Yunhe, Song Jie, Liu Naiyi, Cai Zhengqing, Yulin An, Fang Jie
School of Life Sciences, Anhui University, Hefei, Anhui, PR China.
Nanjing Entry-Exit Inspection and Quarantine Bureau of China, Nanjing, PR China.
Mitochondrial DNA B Resour. 2016 Mar 28;1(1):144-145. doi: 10.1080/23802359.2016.1144101.
The complete mitochondrial genome of (Lepidoptera: Papilionidae) was determined in this study. The mitochondrial genome is a circular molecule of 15 359 and contains 37 genes including 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes and one control region. The nucleotide composition of the mitogenome is strongly biased toward A + T nucleotides (80.45%). Nine protein-coding genes and 14 tRNA genes are encoded on the H strand, and the other four protein-coding genes and eight tRNA genes are encoded on the L strand. The gene order and the orientation of their mitogenomes were similar to all know Papilionidae species. Finally, the phylogenetic relationships of 11 Papilionidae species were reconstructed based on complete mitochondrial genome using the Bayesian inference (BI) and the maximum-likelihood (ML) method. These molecular-based phylogenies support the traditional morphologically based view of relationships within the Papilionidae.
本研究测定了凤蝶科（鳞翅目）某物种的完整线粒体基因组。线粒体基因组是一个15359bp的环状分子，包含37个基因，其中包括13个蛋白质编码基因（PCGs）、22个转运RNA基因、2个核糖体RNA基因和1个控制区。该线粒体基因组的核苷酸组成强烈偏向于A+T核苷酸（80.45%）。9个蛋白质编码基因和14个tRNA基因在重链上编码，另外4个蛋白质编码基因和8个tRNA基因在轻链上编码。其线粒体基因组的基因顺序和方向与所有已知凤蝶科物种相似。最后，基于完整线粒体基因组，使用贝叶斯推断（BI）和最大似然（ML）方法重建了11种凤蝶科物种的系统发育关系。这些基于分子的系统发育支持了凤蝶科内基于传统形态学的关系观点。
