The complete mitochondrial genome of the subsocial cockroach and phylogenomic analyses of Blattodea mitogenomes suggest reclassification of superfamilies.

The genome of the subsocial cockroach was partially sequenced in one-twelfth of an Illumina HiSeq lane. The mitochondrial genome was assembled using MIRA software, yielding a circular molecule of 15,923 bp in length and deposited in GenBank under the accession number KY212743. As expected, the mitogenome contained 13 protein-coding genes, 22 transfer tRNAs and 2 ribosomal RNAs. The molecule was assembled using 35,163 sequencing reads of 120 bp each, resulting in ∼286.9× coverage of uniformly distributed reads along the genome. All the 6 complete mitochondrial genomes available for the roaches from the superfamily Blaberoidea were downloaded and compared with the mitogenome of . We also downloaded complete mitochondrial genomes from the superfamily Blattoidea, including 6 mitochondrial genomes of Termitoidae, 2 mitogenomes of Cryptocercoidae and 3 from Blattoidae. A supermatrix dataset presenting the concatenated alignment of all mitochondrial genes was used as input for a maximum likelihood phylogeny. The phylogenomic tree obtained was consistent for most clades, with a relevant exception in the position of the Corydioidea species . Mitochondrial gene information suggests that superfamily Corydioidea should be classified as a clade inside Blattoidea. Nuclear markers and other Corydioidea mitogenomes should be studied to confirm the evolutionary relationships of Blattodea superfamilies.