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新一代测序技术获得了acc. smg222的完整叶绿体基因组并进行了系统发育分析。

Next-generation sequencing yields the complete chloroplast genome of acc. smg222 and phylogenetic analysis.

作者信息

Wang Heng, Park So-Yeon, Lee Ah-Rim, Jang Seong-Gyu, Im Da-Eun, Jun Tae-Hwan, Lee Joohyun, Chung Jong-Wook, Ham Tae-Ho, Kwon Soon-Wook

机构信息

Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang, Republic of Korea.

Department of Applied Bioscience, Konkuk University, Seoul, Republic of Korea.

出版信息

Mitochondrial DNA B Resour. 2018 Feb 12;3(1):215-216. doi: 10.1080/23802359.2018.1437812.

Abstract

Spring orchid () is one of the most important species belonging to owing to its aesthetic appeal, fragrant flowers and ideal characteristics for using as a houseplant. In this study, the complete chloroplast genome of Korean acc. smg222 was determined by Illumina sequencing. The circular double-stranded DNA of 148,441 bp consisted of two inverted repeat regions of 25,610 bp each, a large single copy region of 83,311 bp, and a small single copy region of 13,910 bp. The genome contained 122 genes, of which 104 were unique and 18 were duplicated within the IRs. The 104 unique genes included 70 protein-coding genes, 30 distinct tRNA genes, and four rRNA genes. Phylogenetic tree analysis revealed that acc. smg222 was clustered with , a cymbidium species native to Korea.

摘要

春兰()因其美学吸引力、芬芳的花朵以及作为室内植物的理想特性,是属于的最重要物种之一。在本研究中,通过Illumina测序确定了韩国春兰acc. smg222的完整叶绿体基因组。148,441 bp的环状双链DNA由两个各为25,610 bp的反向重复区域、一个83,311 bp的大单拷贝区域和一个13,910 bp的小单拷贝区域组成。该基因组包含122个基因,其中104个是独特的,18个在IRs内重复。104个独特基因包括70个蛋白质编码基因、30个不同的tRNA基因和4个rRNA基因。系统发育树分析表明,acc. smg222与韩国本土的大花蕙兰聚在一起。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47c1/7800249/a93230c7062e/TMDN_A_1437812_F0001_B.jpg

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