Key Laboratory for Advanced Materials, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai, 200237, P. R. China.
Bioimaging Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201210, China.
Angew Chem Int Ed Engl. 2021 Apr 6;60(15):8450-8454. doi: 10.1002/anie.202015650. Epub 2021 Mar 3.
Understanding the complex interplay among gasotransmitters is of great significance but remains technically challenging. In this study, we present the design and synthesis of a dually responsive BOD-NH-SC reporter for probing the dynamic and alternating existence of NO and H S in living cells. This designed reporter can repeatedly cycle S-nitrosation and transnitrosation reactions when successively treated with NO and H S, thus affording the interchange of NIR fluorescence at 645 nm (NO) and NIR II fluorescence at 936 nm (H S). In light of this unique fluorescence alternation between two colors, we synthesized water-soluble BOD-NH-SC dots to visualize the intracellular dynamics of NO and H S. These molecular probes thus provide a toolbox to elucidate the interplaying roles of NO and H S in the complex interaction networks of various signal transduction pathways.
理解气体递质之间的复杂相互作用具有重要意义，但在技术上仍然具有挑战性。在本研究中，我们设计并合成了一种双重响应的 BOD-NH-SC 报告分子，用于探测活细胞中 NO 和 H2S 的动态和交替存在。当该报告分子依次与 NO 和 H2S 作用时，可重复进行 S-亚硝化和转亚硝化反应，从而实现近红外荧光（NO，645nm）和近红外二区荧光（H2S，936nm）之间的交替。鉴于这种独特的双色荧光交替，我们合成了水溶性的 BOD-NH-SC 点，以可视化细胞内 NO 和 H2S 的动力学。这些分子探针为阐明 NO 和 H2S 在各种信号转导途径的复杂相互作用网络中相互作用的角色提供了工具包。
