Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai, 200237, P. R. China.
Department of Medicinal Chemistry, School of Pharmacy, Fudan University, Shanghai 201203, P. R. China.
Anal Chem. 2024 Jan 23;96(3):1259-1267. doi: 10.1021/acs.analchem.3c04513. Epub 2024 Jan 11.
The increasing understanding of the intricate relationship between two crucial gasotransmitters nitric oxide (NO) and hydrogen sulfide (HS) in biological actions has generated significant interest. However, comprehensive monitoring of the dynamic fluctuations of endogenous NO and HS remains a challenge. In this study, we have designed an innovative aggregation-induced reporter SAB-NH-SC with enhanced responsiveness to HS for visualizing the fluctuations of intracellular NO and HS. This probe leverages the hydrophilic properties of the pyridinium salt derivative, which can rapidly self-assemble into positively charged nanoparticles under physiological conditions, avoiding the introduction of organic solvents or tedious preparations. Notably, the reporter can repeatedly cycle S-nitrosation and SNO-transnitrosation reactions when successively treated with NO and HS. Consequently, fluorescence alternation at 751 (HS) and 639 nm (NO) facilitates the dynamic visualization of the alternating presence of HS and NO within cells. This dynamic and reversible probe holds immense potential for unraveling the intricate interactions between NO and HS in a complex network of biological applications.
人们对两种关键气体递质一氧化氮（NO）和硫化氢（HS）在生物作用中复杂关系的认识不断加深，这引起了广泛关注。然而，全面监测内源性 NO 和 HS 的动态变化仍然具有挑战性。在本研究中，我们设计了一种创新的聚集诱导报告 SAB-NH-SC，该报告对 HS 具有增强的响应性，可用于可视化细胞内 NO 和 HS 的波动。该探针利用吡啶盐衍生物的亲水性，在生理条件下可迅速自组装成带正电荷的纳米颗粒，避免了有机溶剂的引入或繁琐的制备过程。值得注意的是，当依次用 NO 和 HS 处理时，报告可以反复循环 S-亚硝化和 SNO-转亚硝化反应。因此，在 751nm（HS）和 639nm（NO）处的荧光交替有助于动态可视化细胞内 HS 和 NO 的交替存在。这种动态和可逆的探针在生物应用的复杂网络中揭示 NO 和 HS 之间复杂相互作用方面具有巨大潜力。
