Gillen P, Keeling P, Byrne P J, West A B, Hennessy T P
Department of Surgery, St. James's Hospital, Dublin, Ireland.
Br J Surg. 1988 Feb;75(2):113-5. doi: 10.1002/bjs.1800750208.
Regeneration of canine oesophageal mucosa was studied under basal conditions and in the presence of gastro-oesophageal reflux. In normal circumstances mucosal defects in the oesophagus regenerate by squamous epithelium. In the presence of gastro-oesophageal reflux of either acid or a combination of acid and bile, regeneration was frequently by columnar epithelium (Barrett's oesophagus). This columnar regeneration was not seen with bile reflux alone. By the use of squamous barriers to proximal migration of columnar epithelium in the stomach, it was demonstrated that columnar re-epithelialization may occur from cells intrinsic to the oesophagus and is not dependent on proximal migration of cardiac columnar epithelium. The cell of origin of this epithelium may be located in oesophageal gland ducts and is likely to be a multipotential stem cell since the regenerated columnar epithelium may contain goblet and parietal cells not normally found in the oesophagus. This epithelium is morphologically distinct on mucin histochemistry from cardiac columnar epithelium. These findings support the concept that Barrett's epithelium is metaplastic.
研究了犬食管黏膜在基础条件下以及存在胃食管反流时的再生情况。在正常情况下，食管黏膜缺损由鳞状上皮再生。在存在酸性或酸性与胆汁混合的胃食管反流时，再生通常由柱状上皮完成（巴雷特食管）。单独胆汁反流时未见到这种柱状再生。通过使用鳞状屏障阻止柱状上皮向胃近端迁移，证明柱状上皮再上皮化可能源于食管固有细胞，并不依赖于心形柱状上皮向近端迁移。这种上皮的起源细胞可能位于食管腺导管，很可能是一种多能干细胞，因为再生的柱状上皮可能含有食管中通常不存在的杯状细胞和壁细胞。这种上皮在黏蛋白组织化学上与心形柱状上皮形态不同。这些发现支持巴雷特上皮是化生的这一概念。
