Department of Chemistry, Kandaswami Kandar's College, Namakkal, Tamil Nadu, India.
Department of Microbial Technology, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
Drug Dev Ind Pharm. 2021 Feb;47(2):280-291. doi: 10.1080/03639045.2021.1879835. Epub 2021 Feb 1.
Osteomyelitis is one of the infections of the bone, and the treatment needs to the infection problems. Here, a local therapeutic approach for efficient drug delivery systems was designed to enhance the antibiotic drug's therapeutic activity. Calcium-Alginate nanoparticle (Ca-Alg) crosslinked phosphorylated polyallylamine (PPAA) was prepared through the salting-out technique, and it achieved 82.55% encapsulation of Clindamycin drug. The physicochemical characterizations of FTIR, SEM/EDX, TEM, and XRD were investigated to confirm the materials nature and formation. Clindamycin loaded Ca-Alg/PPAA system showed sustained Clindamycin release from the carrier. Cell viability was assessed in bone-related cells by Trypan blue assay and MTT assay analysis method. Both assay results exhibited better cell viability of synthesized materials against MG63 cells. MIC value of Ca-Alg/PPAA/Clindamycin in the (MRSA) pathogen was 275 µg/mL, and it was 120 µg/mL for pathogen. The materials promising material for Osteomyelitis affected bone regeneration without any destructive effect and speedy recovery of infected parts from these investigations.
骨髓炎是一种骨感染，治疗需要解决感染问题。在这里，设计了一种局部治疗方法来高效传递药物系统，以增强抗生素药物的治疗活性。通过盐析技术制备了钙-海藻酸钠纳米粒子（Ca-Alg）交联的磷酸化聚烯丙胺（PPAA），实现了克林霉素药物 82.55%的包封率。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜/能谱（SEM/EDX）、透射电子显微镜（TEM）和 X 射线衍射（XRD）等物理化学特性进行了研究，以确认材料的性质和形成。克林霉素负载的 Ca-Alg/PPAA 系统显示出载体中克林霉素的持续释放。通过台盼蓝法和 MTT 分析方法评估了骨相关细胞中的细胞活力。两种测定结果均显示合成材料对 MG63 细胞具有更好的细胞活力。Ca-Alg/PPAA/Clindamycin 在耐甲氧西林金黄色葡萄球菌（MRSA）病原体中的 MIC 值为 275μg/mL，而在 病原体中的 MIC 值为 120μg/mL。这些研究表明，该材料有望成为骨髓炎影响骨再生的理想材料，而不会对骨骼造成任何破坏性影响，并能快速恢复受感染部位。
