Niziołek Karina, Słota Dagmara, Sadlik Julia, Łachut Emilia, Florkiewicz Wioletta, Sobczak-Kupiec Agnieszka
Department of Materials Science, Faculty of Materials Engineering and Physics, Cracow University of Technology, 37 Jana Pawła II Av., 31-864 Krakow, Poland.
Materials (Basel). 2023 Sep 27;16(19):6431. doi: 10.3390/ma16196431.
Naturally occurring hydroxyapatite (HA) is the mineral phase of bone tissue. It is characterized by its bioactivity toward stimulating bone cells to proliferate and thus form new apatite layers. For this reason, it is a material commonly used in implantology for filling defects or covering implants (such as endoprostheses). There are several methods to obtain synthetic HA, and by controlling parameters such as temperature, pressure or the drying process, physicochemical parameters of the final powder can be affected. In the present study, HA was obtained by wet precipitation technique and subjected to two different drying methods, determining whether this parameter significantly affects the properties of the final material obtained. Analyzed Fourier-transform infrared spectroscopy (FT-IR) confirmed the presence of functional groups typical for HA. X-ray diffraction analysis (XRD) demonstrated that the materials are partially amorphous; however, the only phase was identified in HA. Scanning electron microscopy (SEM) was used to evaluate the surface morphology and the density, and average grain diameter was measured. Furthermore, HA powders were subjected to modification with the antibiotic clindamycin to determine the potential for use as a carrier for the active substance. The release rate of the drug was determined by high-performance liquid chromatography (HPLC). The differences in the characteristics of the powders were relatively small; however, they affected the rate of drug release from the material as well as the shape of the grains. The method of drying the powders was shown to affect the shape of the grains, as well as the porosity of the sinters prepared from it. A higher amount of clindamycin released into PBS was observed in material with more pores. The materials have demonstrated the potential to be used as a carrier for the active substance; however, further biological, as well as physicochemical, analysis is required.
天然存在的羟基磷灰石（HA）是骨组织的矿物相。其特点是具有生物活性，能刺激骨细胞增殖，从而形成新的磷灰石层。因此，它是植入学中常用于填充缺损或覆盖植入物（如假体）的材料。有几种方法可获得合成HA，通过控制温度、压力或干燥过程等参数，可以影响最终粉末的物理化学参数。在本研究中，通过湿沉淀技术获得HA，并采用两种不同的干燥方法，以确定该参数是否会显著影响所得最终材料的性能。傅里叶变换红外光谱（FT-IR）分析证实了HA典型官能团的存在。X射线衍射分析（XRD）表明材料部分为非晶态；然而，在HA中仅鉴定出一种相。扫描电子显微镜（SEM）用于评估表面形态和密度，并测量平均粒径。此外，对HA粉末用抗生素克林霉素进行改性，以确定其作为活性物质载体的潜力。通过高效液相色谱（HPLC）测定药物的释放速率。粉末特性的差异相对较小；然而，它们影响了药物从材料中的释放速率以及颗粒形状。结果表明，粉末的干燥方法会影响颗粒形状以及由其制备的烧结体的孔隙率。在孔隙较多的材料中观察到更多的克林霉素释放到磷酸盐缓冲液（PBS）中。这些材料已显示出作为活性物质载体的潜力；然而，还需要进一步进行生物学以及物理化学分析。
