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几丁质在多毛孢菌寄生线虫卵过程中调节其基因表达。

Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism.

机构信息

Laboratory of Plant Pathology, Department of Marine Sciences and Applied Biology, University of Alicante, Alicante, 03080, Spain.

Multidisciplinary Institute for Environmental Studies (MIES) Ramon Margalef, University of Alicante, Alicante, 03080, Spain.

出版信息

Environ Microbiol. 2021 Sep;23(9):4980-4997. doi: 10.1111/1462-2920.15408. Epub 2021 Feb 5.

DOI:10.1111/1462-2920.15408
PMID:33496078
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8518118/
Abstract

Climate change makes plant-parasitic nematodes (PPN) an increasing threat to commercial crops. PPN can be managed sustainably by the biocontrol fungus Pochonia chlamydosporia (Pc). Chitosan generated from chitin deacetylation enhances PPN parasitism by Pc. In this work, we investigate the molecular mechanisms of Pc for chitosan resistance and root-knot nematode (RKN) parasitism, using transcriptomics. Chitosan and RKN modify the expression of Pc genes, mainly those involved in oxidation-reduction processes. Both agents significantly modify the expression of genes associated to 113 GO terms and 180 Pc genes. Genes encoding putative glycoproteins (Pc adhesives) to nematode eggshell, as well as genes involved in redox, carbohydrate and lipid metabolism trigger the response to chitosan. We identify genes expressed in both the parasitic and endophytic phases of the Pc lifecycle; these include proteases, chitosanases and transcription factors. Using the Pathogen-Host Interaction database (PHI-base), our previous RNA-seq data and RT-PCR of Pc colonizing banana we have investigated genes expressed both in the parasitic and endophytic phases of Pc lifecycle.

摘要

气候变化使植物寄生线虫（PPN）成为商业作物日益严重的威胁。生物防治真菌蕈状拟青霉（Pc）可以可持续地管理 PPN。壳聚糖脱乙酰作用产生的壳聚糖增强了 Pc 对线虫的寄生能力。在这项工作中，我们使用转录组学研究 Pc 对线虫抗壳聚糖和根结线虫寄生的分子机制。壳聚糖和根结线虫改变 Pc 基因的表达，主要是那些涉及氧化还原过程的基因。这两种因子都显著改变了与 113 个 GO 术语和 180 个 Pc 基因相关的基因的表达。编码对线虫卵壳具有潜在黏附作用的 Pc 基因（Pc 黏附蛋白），以及参与氧化还原、碳水化合物和脂质代谢的基因，触发了对壳聚糖的反应。我们鉴定了在 Pc 生活史的寄生和内生阶段都表达的基因；这些基因包括蛋白酶、壳聚糖酶和转录因子。利用病原体-宿主相互作用数据库（PHI-base）、我们以前的 RNA-seq 数据和 Pc 定殖香蕉的 RT-PCR，我们研究了在 Pc 生活史的寄生和内生阶段都表达的基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/ac55c6d1db0a/EMI-23-4980-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/6919a94c4e6e/EMI-23-4980-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/59802461a2c9/EMI-23-4980-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/15cd662639ae/EMI-23-4980-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/e6c6f7b4a427/EMI-23-4980-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/ac55c6d1db0a/EMI-23-4980-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/6919a94c4e6e/EMI-23-4980-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/59802461a2c9/EMI-23-4980-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/15cd662639ae/EMI-23-4980-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/e6c6f7b4a427/EMI-23-4980-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c7/8518118/ac55c6d1db0a/EMI-23-4980-g005.jpg

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Chitosan Induces Plant Hormones and Defenses in Tomato Root Exudates.壳聚糖诱导番茄根系分泌物中的植物激素并引发防御反应。
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Fungal oxylipins direct programmed developmental switches in filamentous fungi.真菌氧化脂类物质引导丝状真菌中程序性发育转变。
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Chitosan Modulates Volatile Organic Compound Emission from the Biocontrol Fungus .壳聚糖调节生防真菌挥发性有机化合物的排放。
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"Ectomosphere": Insects and Microorganism Interactions.“外质层”：昆虫与微生物的相互作用
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