Department of Environmental Engineering, Yonsei University, Wonju, 26493, Republic of Korea.
J Microbiol. 2021 Mar;59(3):249-258. doi: 10.1007/s12275-021-0590-1. Epub 2021 Jan 26.
Raman spectroscopy is a promising tool for identifying microbial phenotypes based on single cell Raman spectra reflecting cellular biochemical biomolecules. Recent studies using Raman spectroscopy have mainly analyzed phenotypic changes caused by microbial interactions or stress responses (e.g., antibiotics) and evaluated the microbial activity or substrate specificity under a given experimental condition using stable isotopes. Lack of labelling and the nondestructive pretreatment and measurement process of Raman spectroscopy have also aided in the sorting of microbial cells with interesting phenotypes for subsequently conducting physiology experiments through cultivation or genome analysis. In this review, we provide an overview of the principles, advantages, and status of utilization of Raman spectroscopy for studies linking microbial phenotypes and functions. We expect Raman spectroscopy to become a next-generation phenotyping tool that will greatly contribute in enhancing our understanding of microbial functions in natural and engineered systems.
拉曼光谱是一种很有前途的工具，可以根据反映细胞生化生物分子的单细胞拉曼光谱来识别微生物表型。最近使用拉曼光谱的研究主要分析了微生物相互作用或应激反应（例如抗生素）引起的表型变化，并使用稳定同位素评估了给定实验条件下微生物的活性或底物特异性。拉曼光谱缺乏标记以及无损的预处理和测量过程，也有助于对具有有趣表型的微生物细胞进行分类，然后通过培养或基因组分析进行生理学实验。在这篇综述中，我们概述了拉曼光谱用于研究微生物表型和功能联系的原理、优势和利用现状。我们期望拉曼光谱成为下一代表型工具，将极大地促进我们对自然和工程系统中微生物功能的理解。
