Kwon Bo-Ram, Wei Bai, Cha Se-Yeoun, Shang Ke, Zhang Jun-Feng, Kang Min, Jang Hyung-Kwan
Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Center for Poultry Diseases Control, Jeonbuk National University, Iksan 54596, Korea.
Bio Disease Control (BIOD) Co., Ltd., Iksan 54596, Korea.
Animals (Basel). 2021 Jan 20;11(2):246. doi: 10.3390/ani11020246.
The aim of this study was to analyze the prevalence, antimicrobial resistance, and genetic diversity of isolates that were obtained from whole chicken production stages in Korea. A total of 1348 samples were collected from 10 production lines. The prevalence of in breeder farm, broiler farm, slaughterhouse, and retail meat products was 50.0%, 3.3%, 13.4%, and 68.4%, respectively, and was not detected at the hatchery stage. Resistance to quinolones/fluoroquinolones was the most prevalent at all stages. Among the multidrug-resistant isolates, 16 isolates (19.8%) from breeder farm were resistant to both azithromycin and ciprofloxacin. A total of 182 isolates were subdivided into 82 pulsed-field gel electrophoresis (PFGE) genotypes with 100% similarity. Diverse genotypes were presented with discontinuous patterns along the whole production chain. Thirty percent of -free flocks became positive after slaughtering. An identical genotype was simultaneously detected from both breeder farm and retail meat, even from different production lines. This study reveals that antimicrobial-resistant contamination can occur at all stages of the chicken supply chain. In particular, the breeder farm and slaughterhouse should be the main control points, as they are the potential stages at which antimicrobial-resistant could spread to retail meat products by horizontal transmission.
本研究的目的是分析从韩国全鸡生产阶段分离出的菌株的流行情况、抗菌药物耐药性和遗传多样性。从10条生产线共采集了1348份样本。种鸡场、肉鸡场、屠宰场和零售肉类产品中的检出率分别为50.0%、3.3%、13.4%和68.4%，在孵化阶段未检测到。在所有阶段，对喹诺酮类/氟喹诺酮类药物的耐药性最为普遍。在多重耐药菌株中，种鸡场的16株菌株（19.8%）对阿奇霉素和环丙沙星均耐药。总共182株菌株被细分为82种脉冲场凝胶电泳（PFGE）基因型，相似度为100%。在整个生产链中，不同的基因型呈现出不连续的模式。30%的无沙门氏菌鸡群在屠宰后呈阳性。甚至在不同生产线的种鸡场和零售肉类中同时检测到相同的基因型。本研究表明，抗菌药物耐药性沙门氏菌污染可能发生在鸡肉供应链的所有阶段。特别是种鸡场和屠宰场应作为主要控制点，因为它们是抗菌药物耐药性沙门氏菌可能通过水平传播扩散到零售肉类产品的潜在阶段。
