Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, 96010-900 Pelotas, RS, Brazil.
Departamento de Ciência e Tecnologia Agroindustrial, Universidade Federal de Pelotas, 96010-900 Pelotas, RS, Brazil.
Poult Sci. 2019 Feb 1;98(2):932-939. doi: 10.3382/ps/pey365.
Campylobacter is regarded as the most common bacterial cause of gastroenteritis throughout the world and most cases of human campylobacteriosis can be traced back to the consumption of poultry meat. In Brazil, few studies evaluated the genetic relatedness among Campylobacter isolates. The aim of this research was to evaluate the genetic diversity of Campylobacter spp. isolated from poultry meat products sold on the retail market in Southern Brazil. The presumptive identification of Campylobacter was performed using traditional microbiological analysis, followed by molecular confirmation by PCR. The genetic diversity of isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Campylobacter spp. was isolated from 91.7% (33/36) of the samples, totaling 48 isolates. Campylobacter jejuni was the most prevalent species isolated (90.8%). PFGE data revealed 26 pulsotypes and 18 PFGE patterns composed of only 1 isolate. Campylobacter isolates exhibited high genetic diversity; however, some clones were recurrent in the poultry meat products sold on the retail market. As the south region of Brazil is an important producer and exporter of chicken meat, our results highlight the need to control this pathogen in the food chain in this area of the world to reduce the risks of exposing consumers to campylobacteriosis.
空肠弯曲菌被认为是全世界最常见的细菌性胃肠炎致病菌，大多数人类弯曲菌病病例都可追溯到食用禽肉。在巴西，很少有研究评估弯曲菌分离株之间的遗传相关性。本研究旨在评估巴西南部零售市场销售的禽肉产品中分离的弯曲菌 spp 的遗传多样性。采用传统微生物分析进行弯曲菌的初步鉴定，然后通过 PCR 进行分子确证。采用脉冲场凝胶电泳（PFGE）分析分离株的遗传多样性。从 36 个样本中的 91.7%（33/36）分离出弯曲菌，共分离出 48 株。分离出的弯曲菌中最常见的是空肠弯曲菌（90.8%）。PFGE 数据显示有 26 个脉冲型和 18 个仅由 1 个分离株组成的 PFGE 模式。弯曲菌分离株表现出高度的遗传多样性；然而，一些克隆在零售市场销售的禽肉产品中反复出现。由于巴西南部是鸡肉的重要生产和出口地区，我们的研究结果强调了在该地区的食物链中控制这种病原体的必要性，以降低消费者感染弯曲菌病的风险。
