Ladant Daniel
Structural Biology & Chemistry Department, Biochemistry of Macromolecular Interactions Unit, Institut Pasteur CNRS UMR, CEDEX 15, 3528 Paris, France.
Toxins (Basel). 2021 Jan 22;13(2):83. doi: 10.3390/toxins13020083.
The adenylate cyclase toxin, CyaA, is one of the key virulent factors produced by , the causative agent of whooping cough. This toxin primarily targets innate immunity to facilitate bacterial colonization of the respiratory tract. CyaA exhibits several remarkable characteristics that have been exploited for various applications in vaccinology and other biotechnological purposes. CyaA has been engineered as a potent vaccine vehicle to deliver antigens into antigen-presenting cells, while the adenylate cyclase catalytic domain has been used to design a robust genetic assay for monitoring protein-protein interactions in bacteria. These two biotechnological applications are briefly summarized in this chapter.
腺苷酸环化酶毒素（CyaA）是百日咳病原体博德特氏菌产生的关键毒力因子之一。这种毒素主要靶向天然免疫，以促进细菌在呼吸道的定植。CyaA具有几个显著特征，已被用于疫苗学和其他生物技术目的的各种应用。CyaA已被设计成一种有效的疫苗载体，用于将抗原递送至抗原呈递细胞，而腺苷酸环化酶催化结构域已被用于设计一种强大的遗传检测方法，用于监测细菌中的蛋白质-蛋白质相互作用。本章将简要总结这两种生物技术应用。
