Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry (IUSD), Indianapolis, IN 46202, USA.
Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry (IUSD), Indianapolis, IN 46202, USA; Department of Restorative and Prosthetic Dental Sciences, College of Dentistry, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
Dent Mater. 2021 Mar;37(3):508-515. doi: 10.1016/j.dental.2020.12.011. Epub 2021 Jan 23.
To synthesize and characterize a novel resin-based dental material containing 3-aminopropyltriethoxysilane (APTES) surface-modified halloysite-clay nanotubes (HNTs) for long-term delivery of guest molecules.
The optimal concentrations of HNT (10, 15, 20 wt.%) and silane (0, 2, 4 vol.%sil) to be incorporated into the resin-based materials were determined (15 wt.%HNT, 4 vol.%sil) after assessment of the mechanical properties (DC%, degree of conversion; FS, flexural strength; FM, flexural modulus; and UTS, ultimate tensile strength). The HNTsil-powder was loaded with chlorhexidine (CHX) to evaluate the effect of the silanization on drug release. Resin-discs were prepared for the following groups: RES (resin), HNT (resin+15 wt.%HNT), HNTsil (resin+15 wt.%HNT silanized), HNT-CHX (resin+15 wt.%HNT loaded with chlorhexidine), HNTsil-CHX (resin+15 wt.%HNTsil-CHX), and 0.2 vol.%CHX (resin+0.2 vol.%CHX solution). Specimens were stored in water for 1, 3, 5, 10, and 15 days at 37 °C. Aliquots from each time point and the final 15-day specimens were evaluated for the zone of inhibition (ZOI) against Streptococcus mutans. CHX release was analyzed using spectrophotometry at absorbance of 300 nm. Data were statistically analyzed (α = 0.05).
All materials presented similar DC%. Reduced FS but increased FM was detected for 20 wt.%HNT-4%APTES. Groups with 15 wt.% and 20 wt.%HNT with/without APTES presented higher values of UTS. Agar diffusion data indicates that the HNTsil-CHX had a greater ZOI than all other groups over 15 days. HNTsil-CHX had the highest absorbance for day 1 but presented similar values to other groups every time point after.
Silanization of nanotubes followed by encapsulation of chlorhexidine is a promising technique for long-term delivery of guest molecules.
合成并表征一种新型的基于树脂的材料，该材料含有 3-氨丙基三乙氧基硅烷（APTES）表面改性的埃洛石-粘土纳米管（HNTs），用于长期输送客体分子。
通过评估机械性能（DC％，转化率；FS，弯曲强度；FM，弯曲模量；和 UTS，极限拉伸强度），确定要掺入基于树脂的材料中的 HNT（10、15、20wt.％）和硅烷（0、2、4vol.％硅）的最佳浓度（15wt.％HNT，4vol.％硅）。将 HNTsil 粉末加载氯己定（CHX）以评估硅烷化对药物释放的影响。为以下组制备树脂盘：RES（树脂），HNT（树脂+15wt.％HNT），HNTsil（树脂+15wt.％HNT 硅烷化），HNT-CHX（树脂+负载氯己定的 15wt.％HNT），HNTsil-CHX（树脂+负载氯己定的 15wt.％HNTsil）和 0.2vol.％CHX（树脂+0.2vol.％CHX 溶液）。将标本在 37°C 下的水中储存 1、3、5、10 和 15 天。从每个时间点和最终的 15 天标本中取出等分试样，评估对变异链球菌的抑菌区（ZOI）。使用分光光度法在 300nm 处分析 CHX 释放。数据采用统计学方法进行分析（α=0.05）。
所有材料的 DC％均相似。对于 20wt.％HNT-4％APTES，检测到 FS 降低但 FM 增加。具有 15wt.％和 20wt.％HNT 的组，无论是否用 APTES 处理，UTS 值均较高。琼脂扩散数据表明，在 15 天内，HNTsil-CHX 的 ZOI 大于所有其他组。HNTsil-CHX 在第 1 天的吸光度最高，但此后每个时间点的吸光度值与其他组相似。
纳米管的硅烷化随后封装氯己定是一种很有前途的技术，可用于长期输送客体分子。
