载三氯生多孔硅纳米管的实验性正畸粘接剂的聚合、抗菌和生物活性性能。

Polymerisation, antibacterial and bioactivity properties of experimental orthodontic adhesives containing triclosan-loaded halloysite nanotubes.

机构信息

Laboratório de Materiais Dentários, Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, 90035-003, Porto Alegre, Brazil.

Centro Universitário do Distrito Federal (UDF), Brasília, Brazil.

出版信息

J Dent. 2018 Feb;69:77-82. doi: 10.1016/j.jdent.2017.11.002. Epub 2017 Nov 7.

DOI:10.1016/j.jdent.2017.11.002

PMID:29126948

Abstract

OBJECTIVE

To evaluate the immediate enamel bond strength, in situ degree of conversion and the polymerisation rate of three experimental orthodontic adhesives containing triclosan-loaded halloysite nanotubes. The antibacterial and bioactivity properties of such experimental materials were also assessed.

MATERIALS AND METHODS

Three experimental orthodontic adhesives were formulated by incorporating triclosan-loaded halloysite nanotubes (TCN-HNT) at different concentrations (5wt%, 10wt% and 20wt%) into a resin blend (Control). The maximum polymerisation rate of the tested adhesives was evaluated trough FTIR, while Raman was used to analyse the in situ degree of conversion (DC) at the bracket/enamel interface. The shear bond strength (SBS) of the enamel-bonded specimens was assessed at 24h. The antibacterial properties of the experimental materials against S. Mutans were evaluate up to 72h, while, their bioactivity was evaluated after 14days of artificial saliva (AS) storage through SEM-EDS and Raman spectromicroscopy.

RESULTS

Incorporation of TCN-HNT increased the polymerisation properties without interfering with the immediate bonding properties of the experimental adhesives. All experimental adhesives containing TCN-HNT inhibited bacterial growth at 24h, and induced mineral deposition after 14days of AS storage. At 72h, only the experimental system containing 20% TCN-HNT maintained such a capability.

CONCLUSIONS

Adhesives doped with TCN-HNT present improved polymerisation properties and suitable bonding performance. However, only the adhesives containing TCN-HNT >10% might promote long-term antibacterial activity and reliable mineral deposition.

CLINICAL SIGNIFICANCE

The use of adhesives containing triclosan-loaded halloysite represents a promising "smart" approach to bond orthodontic brackets and bands; these might prevent enamel demineralisation and induce enamel remineralisation during the treatment.

摘要

目的

评估三种含有三氯生负载海泡石纳米管的实验性正畸胶粘剂的即刻牙釉质粘结强度、原位转化率和聚合率。还评估了这些实验材料的抗菌和生物活性特性。

材料和方法

通过将三氯生负载海泡石纳米管（TCN-HNT）以不同浓度（5wt%、10wt%和 20wt%）掺入树脂混合物（对照）中，制备了三种实验性正畸胶粘剂。通过傅里叶变换红外光谱（FTIR）评估测试胶粘剂的最大聚合率，而拉曼则用于分析粘结剂/牙釉质界面的原位转化率（DC）。在 24 小时评估牙釉质粘结标本的剪切粘结强度（SBS）。通过在人工唾液（AS）中储存 14 天，通过 SEM-EDS 和拉曼光谱显微镜评估实验材料的抗菌性能，评估了实验材料对 S. Mutans 的抗菌性能，直到 72 小时。