Fundação Oswaldo Cruz-Fiocruz, Instituto Aggeu Magalhães, Recife, PE, Brasil.
Fundação de Medicina Tropical Doutor Heitor Vieira Dourado, Manaus, AM, Brasil.
Mem Inst Oswaldo Cruz. 2021 Jan 25;115:e200339. doi: 10.1590/0074-02760200339. eCollection 2021.
We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From 254 participants with confirmed infection, ZIKV RNA was detected in the sweat of 46 individuals (18.1%). Sweat presented a median cycle threshold (Ct) of 34.74 [interquartile range (IQR) 33.44-36.04], comparable to plasma (Ct 35.96 - IQR 33.29-36.69) and higher than urine (Ct 30.78 - IQR 28.72-33.22). Concomitant detection with other specimens was observed in 33 (72%) of 46 participants who had a positive result in sweat. These findings represent an unusual and not yet investigated virus shedding through eccrine glands.
我们评估了巴西正在进行的队列研究中获得的汗液、血液和尿液标本。在初次皮疹出现后，按照预先设定的时间间隔采集样本，并通过实时逆转录聚合酶链反应（rRT-PCR）检测寨卡病毒（ZIKV）RNA 的存在。在 254 名确诊感染的参与者中，46 人（18.1%）的汗液中检测到 ZIKV RNA。汗液的中位循环阈值（Ct）为 34.74[四分位距（IQR）33.44-36.04]，与血浆（Ct 35.96-IQR 33.29-36.69）相当，高于尿液（Ct 30.78-IQR 28.72-33.22）。在汗液检测呈阳性的 46 名参与者中，有 33 名（72%）同时在其他标本中检测到病毒。这些发现代表了一种不寻常且尚未被研究的通过外分泌腺释放病毒的现象。
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