Lei Xiu Yun, Zhang Ming Ye, Ma Yan Jun, Wang Jian Wen
College of Pharmaceutical Sciences, Soochow University, 215123, Suzhou, China.
J Ind Microbiol Biotechnol. 2017 Oct;44(10):1415-1429. doi: 10.1007/s10295-017-1965-5. Epub 2017 Jul 6.
The addition of surfactant is a useful strategy to enhance the product yield in submerged fermentation process. In this study, we sought to explore the mechanism for the elicitation of Triton X-100 on production of hypocrellin A (HA) in cultures of Shiraia bambusicola through transcriptomic analysis. Triton X-100 at 2.5% (w/v) not only induced HA biosynthesis in mycelia, but also stimulated the release of HA into the medium. We found 23 of 2463 transcripts, possible candidate genes for HA biosynthesis under Triton X-100 induction. Gene ontology (GO) analysis showed Triton X-100 treatment changed expression of genes involved in transmembrane transport and oxidation-reduction process, indicating that enhanced HA production was mainly due to both elicited biosynthesis in mycelium and the increased membrane permeability for HA release. These data provided new insights into elicitation of surfactants in submerged cultures of fungi.
添加表面活性剂是提高深层发酵过程中产品产量的一种有效策略。在本研究中，我们试图通过转录组分析探索曲拉通X-100对竹黄培养物中竹红菌素A（HA）产生的诱导机制。2.5%（w/v）的曲拉通X-100不仅能诱导菌丝体中HA的生物合成，还能刺激HA释放到培养基中。我们在2463个转录本中发现了23个，它们可能是曲拉通X-100诱导下HA生物合成的候选基因。基因本体（GO）分析表明，曲拉通X-100处理改变了参与跨膜运输和氧化还原过程的基因表达，这表明HA产量的提高主要归因于菌丝体中诱导的生物合成以及HA释放时膜通透性的增加。这些数据为真菌深层培养中表面活性剂的诱导作用提供了新的见解。
