Gao Ruijie, Xu Zhecun, Deng Huaxiang, Guan Zhengbing, Liao Xiangru, Zhao Ye, Zheng Xiaohui, Cai Yujie
Key Laboratory of Industrial Biotechnology, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
College of Life Sciences, Northwest University, Xi'an, 710069, Shanxi, China.
Arch Microbiol. 2018 Oct;200(8):1217-1225. doi: 10.1007/s00203-018-1529-8. Epub 2018 Jun 11.
Light is a very important signal for fungi since it influences many different physiological responses. The effects of dark or light at different wavelengths on growth, reproduction and hypocrellins of Shiraia sp. SUPER-H168 were studied: dark, white, red, yellow, green, blue and purple. All incubations under different light conditions had significant stimulating effects on aerial hyphae and suppressing effects on hypocrellin biosynthesis compared with dark incubation. Under blue and purple light especially blue light, the colonies with profuse growth of aerial mycelium were formed. Hypocrellin production reached 13.73 mg per dish under dark condition, and decreased to 4.01 mg and 2.83 mg per dish under white and blue light, respectively. Light condition not only influenced hypocrellin production but also influenced the composition of hypocrellins. Four types of hyphae, namely surface, aerial, biofilm and penetrative hyphae, were observed by light microscopy and SEM. This study found that biofilm hyphae was so closely connected with production of secondary metabolites, and hypocrellins were only produced by biofilm hyphae. Light promoted sexual development and inhibited asexual reproduction, especially blue light strongly inhibited asexual development.
光对真菌来说是一种非常重要的信号，因为它会影响许多不同的生理反应。研究了不同波长的黑暗或光照对竹黄菌SUPER-H168的生长、繁殖和竹红菌素的影响：黑暗、白色、红色、黄色、绿色、蓝色和紫色。与黑暗培养相比，不同光照条件下的所有培养对气生菌丝均有显著的刺激作用，对竹红菌素生物合成有抑制作用。在蓝光和紫光尤其是蓝光下，形成了气生菌丝大量生长的菌落。黑暗条件下竹红菌素产量达到每皿13.73毫克，在白光和蓝光下分别降至每皿4.01毫克和2.83毫克。光照条件不仅影响竹红菌素的产量，还影响竹红菌素的组成。通过光学显微镜和扫描电子显微镜观察到四种类型的菌丝，即表面菌丝、气生菌丝、生物膜菌丝和穿透菌丝。本研究发现生物膜菌丝与次生代谢产物的产生密切相关，竹红菌素仅由生物膜菌丝产生。光照促进有性发育并抑制无性繁殖，尤其是蓝光强烈抑制无性发育。
