Hendrickson Olga D, Byzova Nadezhda A, Zvereva Elena A, Zherdev Anatoly V, Dzantiev Boris B
A.N. Bach Institute of Biochemistry, Federal Research Center "Fundamentals of Biotechnology" of the Russian Academy of Sciences, Leninsky prospect 33, Moscow, Russia 119071.
J Food Sci Technol. 2021 Jan;58(1):292-301. doi: 10.1007/s13197-020-04541-z. Epub 2020 May 29.
Aminoglycosides belong to a class of antibiotics now widely used in agriculture and veterinary medicine and expected to contaminate food products. In this study, a sensitive lateral flow immunoassay (LFIA) of an aminoglycoside neomycin (NEO) was developed. Two methods of immunochromatographic detection based on various techniques of gold nanoparticles (AuNPs) introduction as a label were compared. It was demonstrated that the indirect labeling (a conjugation of anti-species antibodies with a marker) allowed for an increase in assay sensitivity by 80 times. The test system was characterized by an instrumental limit of detection of 0.1 ng/mL and the cutoff level of 10 ng/mL; the assay duration was 15 min. Specificity only toward NEO was demonstrated. The developed LFIA has been tested to detect NEO in different foodstuffs. It has been demonstrated that 70-119% of NEO (coefficients of variations < 10%) can be determined in milk, turkey meat, honey, and eggs using simple procedures of preliminary sample preparation. Testing the samples showed the coincidence of the results for the developed lateral flow assay and for commercial ELISA kit.
氨基糖苷类属于一类目前在农业和兽医学中广泛使用的抗生素，预计会污染食品。在本研究中，开发了一种灵敏的氨基糖苷类新霉素（NEO）的侧向流动免疫分析（LFIA）方法。比较了基于金纳米颗粒（AuNPs）作为标记物引入的各种技术的两种免疫色谱检测方法。结果表明，间接标记（抗物种抗体与标记物的偶联）可使检测灵敏度提高80倍。该测试系统的仪器检测限为0.1 ng/mL，临界值为10 ng/mL；检测时间为15分钟。仅对新霉素表现出特异性。所开发的侧向流动免疫分析法已用于检测不同食品中的新霉素。结果表明，使用简单的样品预处理程序，在牛奶、火鸡肉、蜂蜜和鸡蛋中可测定70-119%的新霉素（变异系数<10%）。对样品的检测表明，所开发的侧向流动分析法与商业酶联免疫吸附测定试剂盒的结果一致。
