Jang Boo-Sik, Lee Mi-Jin, Jeong Noh-Hee
Department of Engineering Chemistry, Chungbuk National University, Cheongu-Si, 28644 Republic of Korea.
CNABiotech., Co., Ltd, Cheongu-Si, Republic of Korea.
J Food Sci Technol. 2021 Jan;58(1):377-382. doi: 10.1007/s13197-020-04586-0. Epub 2020 Jun 23.
The purpose of this study is to examine the possibility of use in various fields such as cosmetics and food industry by extracting, separating, and purifying canola glycoprotein(hreinafter referred to as CNG) and comparing general characteristics and physiological activities with commercially available carrot glycoprotein(hreinafter referred to as CRG). The CNG had a protein content of 13.12%, which is higher than that of common vegetable glycoproteins, and much higher than the CRG of 2.36%. The molecular weight distribution of the CNG was 263-310 Da, which showed a lower molecular weight distribution than the 566-628 Da of the CRG. The total polyphenol content of the CNG was 29.89 mg/g, which was higher than that of the CRG measured at 1.76 mg g. The DPPP radical scavenging activity of CNG and carrot glycoprotein were 10.07 mg mL and 7.76 mg mL, respectively, indicating that CNG had slightly higher electron donating ability than CRG. Total antioxidant activity of CNG was 26.84 mg AA eq/g and CRG was 10.53 mg AA eq/g.
本研究的目的是通过提取、分离和纯化油菜籽糖蛋白（以下简称CNG），并将其一般特性和生理活性与市售胡萝卜糖蛋白（以下简称CRG）进行比较，来研究其在化妆品和食品工业等各个领域的应用可能性。CNG的蛋白质含量为13.12%，高于普通蔬菜糖蛋白，远高于CRG的2.36%。CNG的分子量分布为263 - 310Da，低于CRG的566 - 628Da。CNG的总多酚含量为29.89mg/g，高于CRG测得的1.76mg/g。CNG和胡萝卜糖蛋白的DPPP自由基清除活性分别为10.07mg/mL和7.76mg/mL，表明CNG的供电子能力略高于CRG。CNG的总抗氧化活性为26.84mg AA eq/g，CRG为10.53mg AA eq/g。
