Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil.
Transbound Emerg Dis. 2021 Nov;68(6):3083-3088. doi: 10.1111/tbed.14006. Epub 2021 Feb 21.
The emergence and rapid spread of carbapenemase-producing Enterobacterales represents a serious public health concern. Critically, these global priority bacteria have begun to be reported in companion animals, implying a potential risk of cross-transmission between humans and pets. Using long-read (MinION) and short-read (Illumina) sequencing technologies, we have identified and characterized a hypermucoviscous KPC-2-producing Klebsiella pneumoniae strain belonging to the high-risk international clone ST11/CG258, in a dog with urinary tract infection. Strikingly, the bla gene was carried by a 54-kb IncN plasmid assignated to ST15, which shared 99.8 and 96.8% pairwise identity with IncN-pST15 plasmids from human and environmental K. pneumoniae strains, respectively; all come from an area with high endemicity of KPC-2. Our findings suggest that IncN-pST15 plasmids conferring carbapenem resistance can play as important a role as clonal transmission of K. pneumoniae, representing another major challenge for One Health.
产碳青霉烯酶肠杆菌科的出现和迅速传播是一个严重的公共卫生关注问题。至关重要的是，这些全球优先细菌已开始在伴侣动物中报告，这意味着人类和宠物之间存在交叉传播的潜在风险。我们使用长读（MinION）和短读（Illumina）测序技术，在一只患有尿路感染的狗中鉴定并表征了属于高风险国际克隆 ST11/CG258 的超粘液产 KPC-2 肺炎克雷伯菌株。引人注目的是，bla 基因由一个 54kb 的 IncN 质粒携带，该质粒被分配到 ST15 上，与来自人和环境肺炎克雷伯菌菌株的 IncN-pST15 质粒分别具有 99.8%和 96.8%的成对同一性；所有这些都来自一个碳青霉烯酶-2 高度流行的地区。我们的研究结果表明，赋予碳青霉烯类药物耐药性的 IncN-pST15 质粒可以像肺炎克雷伯菌的克隆传播一样发挥重要作用，这代表了“同一健康”的另一个主要挑战。
