Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Food Res Int. 2021 Jan;139:109956. doi: 10.1016/j.foodres.2020.109956. Epub 2020 Dec 8.
Lactobacillus paracasei FZU103, a probiotic previously isolated from the traditional brewing process of Hongqu rice wine, may have the beneficial effect of improving the disorder of lipid metabolism. This study aimed to determine the beneficial effects of L. paracasei FZU103 on improving hepatic lipid accumulation associated with hyperlipidemia. Results indicated that L. paracasei FZU103 intervention significantly inhibited the abnormal growth of body weight and epididymal white adipose tissue (eWAT), prevented the hypertrophy of epididymal adipocytes, ameliorated the biochemical parameters of serum and liver related to lipid metabolism in HFD-fed mice. Histological analysis also showed that the excessive accumulation oflipid dropletsin the livers induced by HFD-feeding was greatly alleviated by L. paracasei FZU103 intervention. In addition, L. paracasei FZU103 also promoted the excretion of bile acids (BAs) through feces. Metagenomic analysis revealed that oral supplementation with L. paracasei FZU103 significantly increased the relative abundance of Ruminococcus, Alistipes, Pseudoflavonifractor and Helicobacter, but decreased the levels of Blautia, Staphylococcos and Tannerella in HFD-fed mice. The relationships between lipid metabolic parameters and intestinal microbial phylotypes were also revealed by correlation heatmap and network. Furthermore, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS)-based liver metabolomics demonstrated that L. paracasei FZU103 had a significant regulatory effect on the metabolic pathways of glycerophospholipid metabolism, fatty acid degradation, fatty acid elongation, unsaturated fatty acids biosynthesis, riboflavin metabolism, glycerolipid metabolism, primary bile acid biosynthesis, arachidonic acid metabolism, etc. Additionally, L. paracasei FZU103 intervention regulated expression of hepatic genes involved in lipid metabolism and bile acid homeostasis, and promoted fecal excretion of intestinal BAs. These findings present new evidence supporting that L. paracasei FZU103 has the potential to improve lipid metabolism, and could be used as a potential functional food for the prevention of hyperlipidemia.
植物乳杆菌 FZU103 是一种先前从红曲米酒传统酿造过程中分离出来的益生菌，可能具有改善脂质代谢紊乱的有益作用。本研究旨在确定植物乳杆菌 FZU103 改善高脂饮食喂养小鼠肝脏脂质积累相关的脂质代谢紊乱的有益效果。结果表明，植物乳杆菌 FZU103 干预显著抑制了体重和附睾白色脂肪组织（eWAT）的异常生长，防止了附睾脂肪细胞的肥大，改善了高脂饮食喂养小鼠的血清和肝脏与脂质代谢相关的生化参数。组织学分析还表明，高脂饮食喂养引起的肝脏中脂质滴的过度积累通过植物乳杆菌 FZU103 干预得到了极大缓解。此外，植物乳杆菌 FZU103 还通过粪便促进胆汁酸（BAs）的排泄。宏基因组分析显示，口服补充植物乳杆菌 FZU103 显著增加了 Ruminococcus、Alistipes、Pseudoflavonifractor 和 Helicobacter 的相对丰度，但降低了高脂饮食喂养小鼠中 Blautia、Staphylococcos 和 Tannerella 的水平。通过相关热图和网络还揭示了脂质代谢参数与肠道微生物分类群之间的关系。此外，基于超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF/MS）的肝脏代谢组学表明，植物乳杆菌 FZU103 对甘油磷脂代谢、脂肪酸降解、脂肪酸延长、不饱和脂肪酸生物合成、核黄素代谢、甘油脂代谢、初级胆汁酸生物合成、花生四烯酸代谢等代谢途径具有显著的调节作用。此外，植物乳杆菌 FZU103 干预调节了肝脏中参与脂质代谢和胆汁酸稳态的基因表达，并促进了肠道 BAs 的粪便排泄。这些发现为植物乳杆菌 FZU103 改善脂质代谢的潜力提供了新的证据，并可作为预防高脂血症的潜在功能性食品。
