Basic Research & Innovation Division, R&D Unit, AmorePacific Corporation, 1920 Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
Sci Rep. 2021 Jan 28;11(1):2465. doi: 10.1038/s41598-021-81878-1.
Inspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration.
受低强度超声波对组织再生有效性的启发，我们研究了短期高强度超声处理对体外伤口模型和真皮等同物（均包含人真皮成纤维细胞）中伤口愈合加速的潜在影响。不同幅度的短期超声波显著增加了成纤维细胞的增殖和迁移，随后增加了细胞外基质成分纤维连接蛋白和 I 型胶原的产生，这两者对伤口愈合都很重要，并且由成纤维细胞分泌。此外，超声处理增加了嵌入成纤维细胞的三维胶原基质的收缩，并且在 TGF-β存在下协同增加了这种作用。RNA 测序和生物信息学分析显示，超声刺激的成纤维细胞中的基因表达和 p38 和 ERK1/2 MAPK 通路激活发生了变化。我们的研究结果表明，超声作为一种机械刺激可以激活人真皮成纤维细胞。因此，使用超声激活成纤维细胞可能会改善各种类型伤口的愈合并增加皮肤再生。
