Bianco Angelica, Capozzi Loredana, Monno Maria Rosa, Del Sambro Laura, Manzulli Viviana, Pesole Graziano, Loconsole Daniela, Parisi Antonio
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Foggia, Italy.
Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari "Aldo Moro", Bari, Italy.
Front Microbiol. 2021 Jan 12;11:599524. doi: 10.3389/fmicb.2020.599524. eCollection 2020.
Members of the group are spore-forming organisms commonly associated with food poisoning and intestinal infections. Moreover, some strains of the group (i.e., sensu stricto and ) can cause bacteremia in humans, mainly in immunocompromised individuals. Here we performed the genetic characterization of 17 human clinical strains belonging to group isolated from blood culture. The whole-genome sequencing (WGS) revealed that the isolates were closely related to sensu stricto and -type strain. Multilocus sequence typing analysis performed on the draft genome revealed the genetic diversity of our isolates, which were assigned to different sequence types. Based on nucleotide sequence, the isolates were grouped in the phylogenetic groups III and IV. The , , and gene cluster, , , , and , were the most commonly detected virulence genes. Although we did not assess the ability to generate biofilm by phenotypic tests, we verified the prevalence of biofilm associated genes using an approach. A high prevalence of gene cluster, , , , , , , and genes, was found. Genes related to the resistance to penicillin, trimethoprim, and ceftriaxone were identified in most of the isolates. Intriguingly, the majority of these virulence and AMR genes appeared to be evenly distributed among s.s. isolates, as well as closely related to isolates. We showed the WGS represents a good approach to rapidly characterize group strains, being able to give useful information about genetic epidemiology, the presence of virulence and antimicrobial genes, and finally about the potential hazard related to this underestimated risk.
该菌属的成员是形成孢子的生物体，通常与食物中毒和肠道感染有关。此外，该菌属的一些菌株（即狭义的[菌名]和[菌名]）可导致人类菌血症，主要发生在免疫功能低下的个体中。在这里，我们对从血培养中分离出的17株属于该菌属的人类临床菌株进行了基因特征分析。全基因组测序（WGS）显示，这些分离株与狭义的[菌名]和[菌名]型菌株密切相关。对草图基因组进行的多位点序列分型分析揭示了我们分离株的遗传多样性，这些分离株被归为不同的序列类型。基于核苷酸序列，这些分离株被归入系统发育组III和IV。[基因名称]、[基因名称]和[基因名称]基因簇、[基因名称]、[基因名称]、[基因名称]和[基因名称]是最常检测到的毒力基因。虽然我们没有通过表型试验评估生物膜形成能力，但我们使用[方法名称]方法验证了生物膜相关基因的普遍性。发现[基因名称]基因簇、[基因名称]、[基因名称]、[基因名称]、[基因名称]、[基因名称]、[基因名称]和[基因名称]基因的普遍性较高。在大多数分离株中鉴定出了与对青霉素、甲氧苄啶和头孢曲松耐药相关的基因。有趣的是，这些毒力和AMR基因中的大多数似乎在狭义的[菌名]分离株中均匀分布，并且与[菌名]分离株密切相关。我们表明，WGS是快速鉴定该菌属菌株的好方法，能够提供有关遗传流行病学、毒力和抗菌基因的存在以及最终与这种被低估风险相关的潜在危害的有用信息。
