Jang Kyoung-Je, Lee Woong-Sup, Park Sangbae, Han Jinsub, Kim Jae Eun, Kim B Moon, Chung Jong Hoon
Division of Agro-System Engineering, College of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea.
Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Korea.
Nanomaterials (Basel). 2021 Jan 27;11(2):318. doi: 10.3390/nano11020318.
Supramolecular hydrogels are considered promising drug carriers in the tissue engineering field due to their versatile nature. Chitosan hydrogels without chemical cross-linkers have low cytotoxicity and good delivery capacity; however, they have lower mechanical properties for injectable hydrogel usage. In this study, we developed novel chitosan derivatives via click chemistry for fabricating supramolecular hydrogels with higher mechanical strength under mild conditions. The chitosan derivative was successfully synthesized by a sulfur fluoride exchange reaction, and the synthesized chitosan-mPEG/Pluronic-F127 (CS-mPEG/F127) interacted with α-cyclodextrin (α-CD) to form a supramolecular hydrogel via a host-guest reaction. The gelation dynamics, hydrogel properties, and bovine serum albumin (BSA) release could be modulated by the concentration ratio of chitosan-mPEG and F127. This supramolecular hydrogel is a promising protein releasing carrier candidate for long term regeneration therapy.
超分子水凝胶因其多功能性而被认为是组织工程领域中很有前景的药物载体。不含化学交联剂的壳聚糖水凝胶具有低细胞毒性和良好的递送能力；然而，它们的机械性能较低，不适用于可注射水凝胶。在本研究中，我们通过点击化学开发了新型壳聚糖衍生物，以在温和条件下制备具有更高机械强度的超分子水凝胶。通过硫氟交换反应成功合成了壳聚糖衍生物，合成的壳聚糖 - 甲氧基聚乙二醇/普朗尼克 - F127（CS - mPEG/F127）通过主客体反应与α - 环糊精（α - CD）相互作用形成超分子水凝胶。壳聚糖 - 甲氧基聚乙二醇和F127的浓度比可以调节凝胶化动力学、水凝胶性质和牛血清白蛋白（BSA）的释放。这种超分子水凝胶是长期再生治疗中很有前景的蛋白质释放载体候选物。
