Roy Samiron, Akther Mahfuza, Islam Md Sadequl, Meher Mirza Mienur, Sarkar Sumon, Islam Md Shakil, Alam Jahagir, Islam Md Mominul
Department of Pathology and Parasitology, Hajee Mohammad Danesh Science and Technology University, Dinajpue 5200, Bangladesh.
Department of Anatomy and Histology, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh.
Vet Med Int. 2025 Jul 21;2025:4623554. doi: 10.1155/vmi/4623554. eCollection 2025.
Lumpy skin disease (LSD) is an economically important disease of cattle, considered as a threat to the livestock industry. This study aimed to assess the clinical symptoms, gross pathology and histopathology, serum biochemical values, and molecular characterization of LSD in cattle. Cattle in Dinajpur, Thakurgaon, Nilphamari, and Rangpur affected by LSD were initially diagnosed through clinical signs and gross lesions. Blood samples were then collected for biochemical analysis and molecular detection through PCR, and sequencing was performed to characterize the LSD virus (LSDV). The prevalence of LSD in northern areas of Bangladesh was 33.44%. The recorded clinical signs were high fever; firm, raised skin nodules around the head, neck, and limbs; swelling of the limbs and brisket area; and rough hair coat, nasal discharge, dyspnea, corneal opacity, and severe weakness. Grossly, a well-defined, hard swelling of around 1-3.00 cm in diameter in the skin accompanied enlargement of the nearby lymph nodes, and the nodules are composed of a hard, creamy-gray, or yellow clump of the tissue. Histopathologically, ballooning degeneration of the epidermal cell layers, intracytoplasmic eosinophilic inclusion bodies in distinct dermal and epidermal cells, necrosis with substantial infiltration of inflammatory cells, and congested blood vessels in the dermal layer were noted. While total protein, BUN, serum AST, and ALT were increased significantly ( < 0.05), the serum creatinine, GGT, and total albumin were not changed significantly compared with the healthy cattle. Phylogenetic analysis showed that the circulating isolates from northern part of Bangladesh were found in the same clades with India, Pakistan, and Thailand. Thus, this study provides crucial findings on this emerging disease, including its gross and histopathology, serum biochemical properties, and molecular epidemiology in the northern regions of Bangladesh.
牛结节性皮肤病（LSD）是一种对养牛业具有重要经济影响的疾病，被视为对畜牧业的一种威胁。本研究旨在评估牛结节性皮肤病的临床症状、大体病理学和组织病理学、血清生化值以及分子特征。最初通过临床症状和大体病变对迪纳杰布尔、塔库尔冈、尼尔帕马里和朗布尔受牛结节性皮肤病影响的牛进行诊断。然后采集血样进行生化分析，并通过聚合酶链反应（PCR）进行分子检测，同时进行测序以鉴定牛结节性皮肤病病毒（LSDV）。孟加拉国北部地区牛结节性皮肤病的患病率为33.44%。记录到的临床症状有高热；头部、颈部和四肢周围出现坚实、凸起的皮肤结节；四肢和胸部区域肿胀；被毛粗糙、鼻液、呼吸困难、角膜混浊和严重虚弱。大体上，皮肤出现直径约1 - 3.00厘米、边界清晰的硬肿胀，伴有附近淋巴结肿大，结节由坚硬、乳灰色或黄色的组织团块组成。组织病理学检查发现，表皮细胞层出现气球样变性，在真皮和表皮细胞中有胞浆内嗜酸性包涵体，伴有大量炎性细胞浸润的坏死，以及真皮层血管充血。与健康牛相比，总蛋白、血尿素氮、血清谷草转氨酶和谷丙转氨酶显著升高（<0.05），而血清肌酐、γ-谷氨酰转肽酶和总白蛋白无显著变化。系统发育分析表明，孟加拉国北部的流行毒株与印度、巴基斯坦和泰国的毒株属于同一进化枝。因此，本研究提供了关于这种新出现疾病的关键发现，包括其大体和组织病理学、血清生化特性以及孟加拉国北部地区的分子流行病学。
