Molecular and Experimental Mycobacteriology, Research Center Borstel, Leibniz Lung Center, Parkallee 1-40, 23845, Borstel, Germany.
Cellular Microbiology, Research Center Borstel, Leibniz Lung Center, Parkallee 1-40, 23845, Borstel, Germany.
Sci Rep. 2021 Jan 29;11(1):2628. doi: 10.1038/s41598-021-81223-6.
While persistence in a dormant state is crucial for the life cycle of Mycobacterium tuberculosis, no investigation regarding dormancy survival of different strains across different lineages was performed so far. We analyzed responses to oxygen starvation and recovery in terms of growth, metabolism, and transcription. All different strains belonging to the Euro-American lineage (L4) showed similar survival and resuscitation characteristics. Different clinical isolates from the Beijing (L2), East African-Indian (L3), and Delhi/Central Asian (L1) lineage did not survive oxygen starvation. We show that dormancy survival is lineage-dependent. Recovery from O starvation was only observed in strains belonging to the Euro-American (L4) lineage but not in strains belonging to different lineages (L1, L2, L3). Thus, resuscitation from dormancy after oxygen starvation is not a general feature of all M. tuberculosis strains as thought before. Our findings are of key importance to understand infection dynamics of non-Euro-American vs Euro-American strains and to develop drugs targeting the dormant state.
虽然在休眠状态下的持续存在对结核分枝杆菌的生命周期至关重要，但迄今为止，尚未对不同谱系的不同菌株的休眠存活进行调查。我们分析了在生长、代谢和转录方面对缺氧和恢复的反应。属于欧洲-美洲谱系（L4）的所有不同菌株均表现出相似的存活和复苏特征。来自北京（L2）、东非-印度（L3）和德里/中亚（L1）谱系的不同临床分离株不能耐受缺氧。我们表明休眠存活是谱系依赖性的。只有属于欧洲-美洲（L4）谱系的菌株在从 O 饥饿中恢复，而不属于不同谱系（L1、L2、L3）的菌株则没有。因此，与以前的观点相反，从休眠状态复苏并不是所有结核分枝杆菌菌株的普遍特征。我们的研究结果对于理解非欧洲-美洲与欧洲-美洲菌株的感染动态以及开发针对休眠状态的药物具有重要意义。
