Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China.
Guangxi Yangxiang Co., Ltd. Production Center, Guigang, 537131, China.
Sci Rep. 2021 Jan 29;11(1):2622. doi: 10.1038/s41598-021-82126-2.
The difference between the skeletal muscle growth rates of Western and domestic breeds is remarkable, but the potential regulatory mechanism involved is still unclear. Numerous studies have pointed out that long intergenic noncoding RNA (lincRNA) plays a key role in skeletal muscle development. This study used published Yorkshire (LW) and Tibetan pig (TP) transcriptome data to explore the possible role of lincRNA in the difference in skeletal muscle development between the two breeds. 138 differentially expressed lincRNAs (DELs) were obtained between the two breeds, and their potential target genes (PTGs) were predicted. The results of GO and KEGG analysis revealed that PTGs are involved in multiple biological processes and pathways related to muscle development. The quantitative trait loci (QTLs) of DELs were predicted, and the results showed that most QTLs are related to muscle development. Finally, we constructed a co-expression network between muscle development related PTGs (MDRPTGs) and their corresponding DELs on the basis of their expression levels. The expression of DELs was significantly correlated with the corresponding MDRPTGs. Also, multiple MDRPTGs are involved in the key regulatory pathway of muscle fiber hypertrophy, which is the IGF-1-AKT-mTOR pathway. In summary, multiple lincRNAs that may cause differences in skeletal muscle development between the two breeds were identified, and their possible regulatory roles were explored. The findings of this study may provide a valuable reference for further research on the role of lincRNA in skeletal muscle development.
中西方品种骨骼肌生长速率的差异非常显著，但涉及的潜在调控机制尚不清楚。大量研究指出，长链非编码 RNA（lncRNA）在骨骼肌发育中起着关键作用。本研究利用已发表的约克夏猪（LW）和藏猪（TP）转录组数据，探索 lincRNA 在两种猪种骨骼肌发育差异中的可能作用。在两个品种之间获得了 138 个差异表达的 lincRNA（DEL），并预测了它们的潜在靶基因（PTGs）。GO 和 KEGG 分析的结果表明，PTGs 参与了多个与肌肉发育相关的生物学过程和途径。预测了 DEL 的数量性状位点（QTLs），结果表明大多数 QTLs 与肌肉发育有关。最后，我们根据其表达水平，在与肌肉发育相关的 PTGs（MDRPTGs）及其相应的 DEL 之间构建了一个共表达网络。DEL 的表达与相应的 MDRPTGs 显著相关。此外，多个 MDRPTGs 参与了肌肉纤维肥大的关键调控途径，即 IGF-1-AKT-mTOR 途径。总之，鉴定出了多个可能导致两个品种骨骼肌发育差异的 lincRNA，并探讨了它们的潜在调控作用。本研究的结果可能为进一步研究 lincRNA 在骨骼肌发育中的作用提供有价值的参考。
