Lee Ji-Heun, Zhang Didi, Kwak Seong-Eun, Shin Hyung-Eun, Song Wook
Institute of Sports Science, Department of Physical Education, Seoul National University, Seoul, Korea.
Institute on Aging, Seoul National University, Seoul, Korea.
J Obes Metab Syndr. 2021 Mar 30;30(1):44-54. doi: 10.7570/jomes20043.
Exercise and high fat, high sucrose restriction diets are well known treatments for obesity. The aim of this study was to measure the effects of those lifestyle interventions on molecular transducers of exercise, such as Nr4a3, mitochondria-associated proteins, and muscle function.
We conducted 8 weeks of treadmill exercise and sucrose or fat restriction diets in obese mice. The mice were divided into eight groups: the normal diet (CON) group, normal diet with exercise (CONEX) group, high fat, high sucrose diet (HFHS) group, HFHS with exercise (HFHSEX) group, sucrose restriction (SR) group, SR with exercise (SREX) group, high fat, high sucrose restriction (ND) group, and ND with exercise (NDEX) group.
The 8 weeks of exercise reduced body weight, improved lipid profiles (total cholesterol, triglycerides), and increased hanging time. The combination of exercise and a fat and sucrose restriction diet improved glucose tolerance and increased grip strength. The 8 weeks of intervention did not significantly affect the Nr4a3 protein level. The sucrose and fat restriction diet increased the phosphorylated protein kinase B (pAkt)/Akt ratio, and its level was lower in the HFHS group. Exercise increased the protein expression level of PGC-1α in obese conditions. Moreover, SR led reduced the phosphorylated AMP-activated protein kinase (pAMPK)/AMPK ratio and PGC-1α to the control level.
The 8 weeks of exercise or a sucrose and fat restriction diet improved metabolic indicators and muscle function. SR reduced pAMPK/AMPK and PGC-1α to the control level. Nr4a3 protein expression was not significantly changed by either exercise or a fat and sucrose restriction diet.
运动以及高脂、高蔗糖限制饮食是治疗肥胖症的常用方法。本研究旨在测定这些生活方式干预对运动分子传感器（如Nr4a3、线粒体相关蛋白和肌肉功能）的影响。
我们对肥胖小鼠进行了8周的跑步机运动以及蔗糖或脂肪限制饮食。小鼠被分为八组：正常饮食（CON）组、正常饮食加运动（CONEX）组、高脂高蔗糖饮食（HFHS）组、高脂高蔗糖饮食加运动（HFHSEX）组、蔗糖限制（SR）组、蔗糖限制加运动（SREX）组、高脂高蔗糖限制（ND）组和高脂高蔗糖限制加运动（NDEX）组。
8周的运动减轻了体重，改善了血脂水平（总胆固醇、甘油三酯），并延长了悬挂时间。运动与脂肪和蔗糖限制饮食相结合改善了葡萄糖耐量并增强了握力。8周的干预对Nr4a3蛋白水平没有显著影响。蔗糖和脂肪限制饮食增加了磷酸化蛋白激酶B（pAkt）/Akt的比率，且该水平在HFHS组中较低。运动增加了肥胖状态下PGC-1α的蛋白表达水平。此外，蔗糖限制使磷酸化AMP激活蛋白激酶（pAMPK）/AMPK的比率和PGC-1α降至对照水平。
8周的运动或蔗糖和脂肪限制饮食改善了代谢指标和肌肉功能。蔗糖限制使pAMPK/AMPK和PGC-1α降至对照水平。运动或脂肪和蔗糖限制饮食均未显著改变Nr4a3蛋白表达。
