Zhang Feng, Wu Shi, Huang Jiahui, Yang Runshi, Zhang Jumei, Lei Tao, Dai Jingsha, Ding Yu, Xue Liang, Wang Juan, Chen Moutong, Wu Qingping
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
Front Microbiol. 2021 Jan 15;11:598990. doi: 10.3389/fmicb.2020.598990. eCollection 2020.
Antimicrobial resistance has become a major public health threat. Food-related species have received much attention due to their multidrug resistance. The gene associated with multidrug resistance has been consistently detected in food-derived species. In this retrospective study, we examined the prevalence of -positive strains isolated from poultry meat in different geographical areas of China from 2011 to 2016. Two -positive strains were identified from poultry meat in China. Comparative and whole-genome analyses were performed to characterize the genetic features and overall antimicrobial resistance genes in the two isolates 245-1 and 2794-1. Whole-genome sequencing showed that they both harbored a novel 20,258-bp -carrying Tn transposon derivative on their chromosomes. The Tn derivative harbors multiple antimicrobial resistance genes, including the transferable multiresistance gene , chloramphenicol resistance gene , aminoglycoside resistance genes and , and bleomycin resistance gene . Surprisingly, within the Tn derivative, an active unconventional circularizable structure containing various resistance genes and a copy of a direct repeat sequence was identified by two-step PCR. Furthermore, core genome phylogenetic analysis revealed that the -positive strains were most closely related to 14S03313-1 isolated from Japan in 2017 and 14S03319-1 isolated from Switzerland in 2017. This study is the first report of harboring a novel -carrying Tn558 derivative isolated from retail food. This finding raises further concerns regarding the potential threat to food safety and public health safety. The occurrence and dissemination of similar -carrying transposons from diverse species need further surveillance.
抗菌药物耐药性已成为主要的公共卫生威胁。与食品相关的物种因其多重耐药性而备受关注。在源自食品的物种中一直检测到与多重耐药性相关的基因。在这项回顾性研究中，我们调查了2011年至2016年中国不同地理区域从禽肉中分离出的阳性菌株的流行情况。在中国的禽肉中鉴定出两株阳性菌株。对两株分离株245 - 1和2794 - 1进行了比较分析和全基因组分析，以表征其遗传特征和总体抗菌药物耐药基因。全基因组测序表明，它们的染色体上均携带一个新的携带20,258 bp的转座子衍生物。该转座子衍生物含有多个抗菌药物耐药基因，包括可转移的多耐药基因、氯霉素耐药基因、氨基糖苷类耐药基因和以及博来霉素耐药基因。令人惊讶的是，在该转座子衍生物中，通过两步PCR鉴定出一个含有各种耐药基因和一个直接重复序列拷贝的活跃的非常规可环化结构。此外，核心基因组系统发育分析表明，阳性菌株与2017年从日本分离出的14S03313 - 1和2017年从瑞士分离出的14S03319 - 1关系最为密切。本研究首次报道了从零售食品中分离出携带新型转座子衍生物的情况。这一发现进一步引发了对食品安全和公共卫生安全潜在威胁的担忧。来自不同物种的类似携带转座子的出现和传播需要进一步监测。
