Jaiswal Damini, Sidharthan V Kavi, Sharma Susheel Kumar, Rai Richa, Choudhary Nandlal, Ghosh Amalendu, Baranwal Virendra Kumar
Advanced Centre for Plant Virology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.
ICAR-Research Complex for NEH Region, Manipur Centre, Imphal, 795004 India.
3 Biotech. 2021 Feb;11(2):88. doi: 10.1007/s13205-021-02641-x. Epub 2021 Jan 22.
Citrus greening disease or huanglongbing (HLB) caused by Liberibacter asiaticus (Las) limits citrus production worldwide. Las is transmitted by the Asian citrus psyllid (ACP), (Hemiptera: Psyllidae) in a persistent-propagative manner. Understanding the molecular interaction between Las and ACP and interrupting the interrelationship can provide an alternative to insecticides for managing citrus greening disease. Transcriptome analysis of ACP in response to Las showed differential expression of 3911 genes (2196 upregulated, and 1715 downregulated) including the key genes of ACP involved in cytoskeleton synthesis and nutrition-related proteins, such as vitellogenins, extensin, laminin, tropomyosin, troponin C, and flightin. Majority of the differentially expressed genes were categorized under molecular functions followed by cellular components and biological processes. KEGG pathway analysis showed differential regulation of carbohydrate, nucleotide, and energy metabolic pathways, the endocytotic pathway, and the defense-related pathways. Differential regulation of genes associated with the key pathways might favour Las to become systemic and propagate in its insect vector. The study provides an understanding of genes involved in circulation of Las in ACP. The candidate genes involved in key physiological processes and Las transmission by ACP would be potential targets for sustainable management of ACP and Las.
The online version contains supplementary material available at 10.1007/s13205-021-02641-x.
由亚洲韧皮杆菌（Las）引起的柑橘黄龙病（HLB）限制了全球柑橘产量。Las以持久性增殖方式由亚洲柑橘木虱（ACP）（半翅目：木虱科）传播。了解Las与ACP之间的分子相互作用并中断这种相互关系可为管理柑橘黄龙病提供一种替代杀虫剂的方法。对响应Las的ACP进行转录组分析显示，3911个基因表达存在差异（2196个上调，1715个下调），包括参与细胞骨架合成和营养相关蛋白（如卵黄原蛋白、伸展蛋白、层粘连蛋白、原肌球蛋白、肌钙蛋白C和飞行肌动蛋白）的ACP关键基因。大多数差异表达基因归类于分子功能，其次是细胞成分和生物学过程。KEGG通路分析显示碳水化合物、核苷酸和能量代谢通路、内吞通路以及防御相关通路存在差异调节。与关键通路相关的基因差异调节可能有利于Las在其昆虫载体中成为系统性并进行传播。该研究有助于了解参与Las在ACP中循环的基因。参与关键生理过程以及Las通过ACP传播的候选基因将是可持续管理ACP和Las的潜在靶点。
在线版本包含可在10.1007/s13205-021-02641-x获取的补充材料。
