Division of Clinical and Molecular Allergology, Research Center Borstel, Airway Research Center North (ARCN), German Center for Lung Research, 23845 Borstel, Germany.
Interdisciplinary Allergy Outpatient Clinic, Department of Internal Medicine and Pneumology, University of Lübeck, 23538 Lübeck, Germany.
Nutrients. 2021 Jan 28;13(2):409. doi: 10.3390/nu13020409.
Lupine flour is a valuable food due to its favorable nutritional properties. In spite of its allergenic potential, its use is increasing. Three lupine species, , , and . are relevant for human nutrition. The aim of this study is to clarify whether the species differ with regard to their allergen composition and whether anaphylaxis marker allergens could be identified in lupine. Patients with the following characteristics were included: lupine allergy, suspected lupine allergy, lupine sensitization only, and peanut allergy. Lupine sensitization was detected via CAP-FEIA (ImmunoCAP) and skin prick test. Protein, DNA and expressed sequence tag (EST) databases were queried for lupine proteins homologous to already known legume allergens. Different extraction methods applied on seeds from all species were examined by SDS-PAGE and screened by immunoblotting for IgE-binding proteins. The extracts underwent different and successive chromatography methods. Low-molecular-weight components were purified and investigated for IgE-reactivity. Proteomics revealed a molecular diversity of the three species, which was confirmed when investigated for IgE-reactivity. Three new allergens, profilin, and . lipid transfer protein (LTP), were identified. LTP as a potential marker allergen for severity is a valuable additional candidate for molecular allergy diagnostic tests.
羽扇豆粉因其良好的营养特性而成为一种有价值的食品。尽管它具有致敏性，但它的使用正在增加。有三种羽扇豆， ， 和 ，对人类营养很重要。本研究的目的是阐明这些物种在过敏原组成上是否存在差异，以及是否可以在羽扇豆中鉴定出过敏反应标志物过敏原。纳入具有以下特征的患者：羽扇豆过敏、疑似羽扇豆过敏、仅羽扇豆致敏和花生过敏。通过 CAP-FEIA（免疫 CAP）和皮肤点刺试验检测羽扇豆致敏。针对与已知豆科过敏原同源的羽扇豆蛋白，对蛋白质、DNA 和表达序列标签 (EST) 数据库进行了查询。对所有物种的种子进行了不同的提取方法，并通过 SDS-PAGE 进行了检查，并通过免疫印迹进行了 IgE 结合蛋白的筛选。提取物经历了不同的连续层析方法。对低分子量成分进行了纯化，并研究了 IgE 反应性。蛋白质组学揭示了三种羽扇豆的分子多样性，当研究其 IgE 反应性时得到了证实。鉴定出三种新的过敏原，即 亲环素、 和. 脂质转移蛋白 (LTP)。LTP 作为严重程度的潜在标志物过敏原，是分子过敏诊断测试的有价值的附加候选物。
