Centre of Space Biomedicine, Department of Systems Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
Department of Industrial Engineering, University of Rome Tor Vergata, 00133 Rome, Italy.
Int J Mol Sci. 2021 Jan 28;22(3):1321. doi: 10.3390/ijms22031321.
Flavonoids display a broad range of structures and are responsible for the major organoleptic characteristics of plant-derived foods and beverages. Recent data showed their activity, and in particular of luteolin-7-O-glucoside (LUT-7G), in reduction of oxidative stress and inflammatory mechanisms in different physiological systems. In this paper, we tried to elucidate how LUT-7G could exert both antioxidant and anti-inflammatory effects in endothelial cells cultured in vitro. Here, we showed that LUT-7G is able to inhibit the STAT3 pathway, to have an antiproliferative action, and an important antioxidant property in HUVEC cells. These properties are exerted by the flavone in endothelial through the transcriptional repression of a number of inflammatory cytokines and their receptors, and by the inhibition of ROS generation. ROS and STAT3 activation has been correlated with the production of oxysterols and other hydroxylated fatty acids, and they have been recognized important as players of atherogenesis and cardiocirculatory system diseases. The analysis of the general production pathway of these hydroxylated species, showed a strong decrease of cholesterol hydroxylated species such as 7-alpha-hydroxicholesterol, 7-beta-hydroxicholesterol by the treatment with LUT-7G. This confirms the anti-inflammatory properties of LUT-7G also in the endothelial district, showing for the first time the molecular pathway that verify previous postulated cardiovascular benefits of this flavone.
类黄酮呈现出广泛的结构，是植物源性食品和饮料主要感官特性的主要原因。最近的数据表明，它们具有活性，特别是木犀草素-7-O-葡萄糖苷（LUT-7G），可减少不同生理系统中的氧化应激和炎症机制。在本文中，我们试图阐明 LUT-7G 如何在体外培养的内皮细胞中发挥抗氧化和抗炎作用。在这里，我们表明 LUT-7G 能够抑制 STAT3 途径，具有抗增殖作用，并且在 HUVEC 细胞中具有重要的抗氧化特性。这些特性是通过在内皮细胞中抑制转录，从而抑制多种炎症细胞因子及其受体的表达，以及抑制 ROS 的产生来发挥的。ROS 和 STAT3 的激活与氧化固醇和其他羟化脂肪酸的产生有关，它们已被认为是动脉粥样硬化和心血管系统疾病的重要参与者。对这些羟化物质的一般产生途径进行分析，表明 LUT-7G 处理后胆固醇羟化物质（如 7-α-羟胆固醇、7-β-羟胆固醇）的产量明显下降。这证实了 LUT-7G 在血管内皮区也具有抗炎作用，首次证明了验证先前假设的这种黄酮类化合物对心血管益处的分子途径。
