Sara El Asri, Reda Ben Mrid, Zakia Zouaoui, Bitwell Chibuye, Abdelhamid Ennoury, Imad Kabach, Mohamed Nhiri, Fatiha Chibi
Laboratory of Biochemistry and Molecular Genetics, Faculty of Sciences and Technology of Tangier, Abdelmalek Essaadi University, Tetouan, Morocco.
Institute of Biological Sciences (ISSB-P), Faculty of Medical Sciences (FMS), Mohammed VI Polytechnic University (UM6P), Ben-Guerir, Morocco.
Int J Environ Health Res. 2025 Jul;35(7):1739-1751. doi: 10.1080/09603123.2024.2404248. Epub 2024 Sep 18.
The present study evaluated a range of biological activities of leaves, often considered as by-products, in relation to hyperglycemia. The antiglycation potential of this plant has not been previously reported. In this research, the methanolic leaf extract of was assessed for its antioxidant, enzyme inhibitory and antiglycation activities. The bioactive compounds present in the extract were screened using LC-MS/MS. Enzyme inhibitory activities were tested on α-glucosidase and α-amylase, and the antiglycation effect was investigated using BSA-fructose model. The methanolic extract showed a high polyphenolic contents (176.26 ± 2.26 mg GAE/g) and important IC values for DPPH (34.79 ± 2.40 µg/mL) and ABTS radical scavenging activities (49.75 ± 2.47 µg/mL). In addition, the plant leaf extract significantly inhibited hyperglycemia-related enzymes in a dose-dependent manner and demonstrated a reduction in fluorescent AGEs, fructosamine, and dicarbonyl compounds. Therefore, cv could be an effective source of therapeutics for improving the healthcare outcomes of diabetic patients.
本研究评估了通常被视为副产品的叶子与高血糖相关的一系列生物活性。此前尚未报道过这种植物的抗糖化潜力。在本研究中，对该植物叶子的甲醇提取物进行了抗氧化、酶抑制和抗糖化活性评估。使用液相色谱-串联质谱法（LC-MS/MS）对提取物中存在的生物活性化合物进行了筛选。对α-葡萄糖苷酶和α-淀粉酶进行了酶抑制活性测试，并使用牛血清白蛋白-果糖模型研究了抗糖化作用。甲醇提取物显示出高多酚含量（176.26±2.26毫克没食子酸当量/克），对二苯基苦味酰基自由基（DPPH）（34.79±2.40微克/毫升）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基清除活性具有重要的半数抑制浓度（IC）值（49.75±2.47微克/毫升）。此外，该植物叶子提取物以剂量依赖的方式显著抑制了与高血糖相关的酶，并显示出荧光晚期糖基化终产物（AGEs）、果糖胺和二羰基化合物的减少。因此，该植物品种可能是改善糖尿病患者健康状况的有效治疗来源。
