Phytochemical Profiling of Methanolic Fruit Extract of Ait. by LC-MS/MS Analysis and Evaluation of Its Antioxidant and Antimicrobial Activity.

Ait. (Rubiaceae) is also known as Indian Boxwood is a small deciduous tree often growing in southern states of India. In the present study, phytochemical profiling of methanolic extract of fruits were carried out using FTIR and LC-MS/MS analysis. Besides, its antioxidant and antimicrobial potential have been analysed using DPPH activity, differential pulse voltammetry and resazurin microtiter assay, respectively. Phytochemical profiling revealed the presence of 22 major diversified compounds and main were 3-caffeoyl quinic acid (chlorogenic acid), 3,4-Di-O-caffeoyl quinic acid, 6-O-trans-feruloylgenipin gentiobioside, 10-(6-O-trans sinapoyl glucopyranosyl) gardendiol, isoquercitrin, scortechinones, secaubryenol, iridoids and quercetin 3-rutinoside (rutin). The extract showed antioxidant activity (IC = 65.82) and powerful antibacterial activity with lowest minimum inhibitory concentration against Gram-positive (15.62 µg/µL), (31.25 µg/µL) than gram negative (62.5 µg/µL), (62.5 µg/µL), (31.25 µg/µL). This study shows that the fruits of have tremendous potential to be used in food industries, phyto-therapeutics and cosmetic industries.