Berthold Erin C, Yang Rui, Sharma Abhisheak, Kamble Shyam H, Kanumuri Siva R, King Tamara I, Popa Raluca, Freeman Joshua H, Brym Zachary T, Avery Bonnie A, McCurdy Christopher R
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL, USA.
North Florida Research and Education Center, University of Florida, Quincy, FL, USA.
J Cannabis Res. 2020 Dec 10;2(1):42. doi: 10.1186/s42238-020-00050-0.
In 2018, the Farm Bill mandated the United States Department of Agriculture to develop regulations governing the cultivation, processing, and marketing of industrial hemp. Industrial hemp is defined as Cannabis sativa L. with a total Δ-9-tetrahydrocannabinol (Δ-9-THC) content ≤0.3%. Therefore, for hemp to become an agricultural commodity, it is important to regulate production by developing standard methods for sampling and testing of the plant material.
An ultra-performance liquid chromatography-tandem mass spectrometry analytical method for the quantification of twelve cannabinoids was developed. The method was applied to a regulatory sampling trial of three hemp varieties cultivated for cannabidiol (CBD) production. Two samples were taken from 28 plants with one sample being flower only while the other was a composite sample that included flowers, leaves, and stems.
The assay method was validated for specificity, range, repeatability, reproducibility, and recovery in accordance with all applicable standards for analytical methods. The results of the regulatory study indicated a significant decrease in the concentration of total Δ-9-THC and total CBD of 0.09% and 1.32%, respectively, between a flower only and a composite sample.
There are many factors that may influence reported total Δ-9-THC content in industrial hemp. A robust analytical method was developed to analyze hemp samples in a trial regulatory study. The results indicate that the way hemp is sampled and analyzed may influence the legality of a crop, which could have negative economic and legal consequences.
2018年，《农业法案》授权美国农业部制定有关工业大麻种植、加工和销售的法规。工业大麻被定义为Δ-9-四氢大麻酚（Δ-9-THC）总含量≤0.3%的大麻。因此，为使大麻成为一种农产品，通过制定植物材料的采样和检测标准方法来规范生产很重要。
开发了一种用于定量测定12种大麻素的超高效液相色谱-串联质谱分析方法。该方法应用于一项针对为生产大麻二酚（CBD）而种植的三个大麻品种的监管采样试验。从28株植物上采集了两个样本，一个样本仅为花朵，另一个是包含花朵、叶子和茎的混合样本。
根据所有适用的分析方法标准，对该检测方法的特异性、范围、重复性、再现性和回收率进行了验证。监管研究结果表明，仅花朵样本和混合样本之间，Δ-9-THC总浓度和CBD总浓度分别显著降低了0.09%和1.32%。
有许多因素可能会影响工业大麻中报告的Δ-9-THC总含量。在一项试验性监管研究中开发了一种可靠的分析方法来分析大麻样本。结果表明，大麻的采样和分析方式可能会影响作物的合法性，这可能会产生负面的经济和法律后果。
