Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland.
Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Molecules. 2021 Jan 26;26(3):631. doi: 10.3390/molecules26030631.
The species (lungwort) are edible plants and traditional remedies for different disorders of the respiratory system. Our work covers a comparative study on biological actions in human blood plasma and cyclooxygenase-2 (COX-2) -inhibitory properties of plant extracts (i.e., phenolic-rich fractions) originated from aerial parts of Dumort. and L. Phytochemical profiling demonstrated the abundance of phenolic acids and their derivatives (over 80% of the isolated fractions). Danshensu conjugates with caffeic acid, i.e., rosmarinic, lithospermic, salvianolic, monardic, shimobashiric and yunnaneic acids were identified as predominant components. The examined extracts (1-100 µg/mL) partly prevented harmful effects of the peroxynitrite-induced oxidative stress in blood plasma (decreased oxidative damage to blood plasma components and improved its non-enzymatic antioxidant capacity). The cellular safety of the extracts was confirmed in experimental models of blood platelets and peripheral blood mononuclear cells. COX-2 inhibitor screening evidently suggested a stronger activity of (IC of 13.28 and 7.24 µg/mL, in reaction with synthetic chromogen and physiological substrate (arachidonic acid), respectively). In silico studies on interactions of main components of the extracts with the COX-2 demonstrated the abilities of ten compounds to bind with the enzyme, including rosmarinic acid, menisdaurin, globoidnan A and salvianolic acid H.
该物种（肺草）是可食用植物，也是治疗呼吸系统不同疾病的传统药物。我们的工作包括对人血浆中的生物活性和植物提取物（即富含酚类的部分）的环氧化酶-2（COX-2）抑制特性进行比较研究，这些植物提取物来源于 Dumort. 和 L. 的地上部分。植物化学分析表明，酚酸及其衍生物（占分离部分的 80%以上）含量丰富。丹酚酸与咖啡酸的结合物，即迷迭香酸、石竹烯酸、丹参酸、单咖啡酰酒石酸、紫草酸和云南酸，被鉴定为主要成分。研究中的提取物（1-100μg/mL）部分预防了过氧亚硝酸盐诱导的血浆氧化应激的有害影响（降低了对血浆成分的氧化损伤，提高了其非酶抗氧化能力）。在血小板和外周血单核细胞的实验模型中，证实了提取物的细胞安全性。COX-2 抑制剂筛选明显表明 （IC 为 13.28 和 7.24μg/mL，分别与合成显色剂和生理底物（花生四烯酸）反应）具有更强的活性。对 提取物主要成分与 COX-2 相互作用的计算机模拟研究表明，有 10 种化合物具有与酶结合的能力，其中包括迷迭香酸、menisdaurin、globoidnan A 和丹参酸 H。
