University Center of Orthopedic, Trauma and Plastic Surgery, University Hospital Carl Gustav Carus at TU Dresden, 01307 Dresden, Germany.
Center for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus at TU Dresden, 01307 Dresden, Germany.
Int J Mol Sci. 2022 Mar 21;23(6):3400. doi: 10.3390/ijms23063400.
To treat critical-size bone defects, composite materials and tissue-engineered bone grafts play important roles in bone repair materials. The purpose of this study was to investigate the bone regenerative potential of hybrid scaffolds consisting of macroporous calcium phosphate cement (CPC) and microporous mineralized collagen matrix (MCM). Hybrid scaffolds were synthetized by 3D plotting CPC and then filling with MCM (MCM-CPC group) and implanted into a 5 mm critical size femoral defect in rats. Defects left empty (control group) as well as defects treated with scaffolds made of CPC only (CPC group) and MCM only (MCM group) served as controls. Eight weeks after surgery, micro-computed tomography scans and histological analysis were performed to analyze the newly formed bone, the degree of defect healing and the activity of osteoclasts. Mechanical stability was tested by 3-point-bending of the explanted femora. Compared with the other groups, more newly formed bone was found within MCM-CPC scaffolds. The new bone tissue had a clamp-like structure which was fully connected to the hybrid scaffolds and thereby enhanced the biomechanical strength. Together, the biomimetic hybrid MCM-CPC scaffolds enhanced bone defect healing by improved osseointegration and their differentiated degradation provides spatial effects in the process of critical-bone defect healing.
为了治疗临界尺寸的骨缺损，复合材料和组织工程化骨移植物在骨修复材料中起着重要作用。本研究旨在研究由大孔磷酸钙水泥（CPC）和微孔矿化胶原基质（MCM）组成的混合支架的骨再生潜力。通过 3D 绘图 CPC 合成混合支架，然后用 MCM 填充（MCM-CPC 组）并植入大鼠 5mm 临界尺寸股骨缺损。留下空缺陷（对照组）以及仅用 CPC 制成的支架（CPC 组）和仅用 MCM 处理的支架（MCM 组）作为对照。手术后 8 周，进行微计算机断层扫描和组织学分析，以分析新形成的骨、缺损愈合程度和破骨细胞的活性。通过对植入的股骨进行三点弯曲测试来测试机械稳定性。与其他组相比，在 MCM-CPC 支架中发现了更多的新形成骨。新的骨组织具有夹状结构，与混合支架完全相连，从而增强了生物力学强度。仿生混合 MCM-CPC 支架通过改善骨整合增强了骨缺损愈合，其差异化降解在临界骨缺损愈合过程中提供了空间效应。
