Department of Pathology, New York Medical College, Valhalla, NY, 10595, USA.
Department Biophysics and Physiology, Faculty of Biology, University of Bucharest, Bucharest, Romania.
Sci Rep. 2021 Feb 2;11(1):2743. doi: 10.1038/s41598-021-82383-1.
Myocardium transcriptomes of left and right atria and ventricles from four adult male C57Bl/6j mice were profiled with Agilent microarrays to identify the differences responsible for the distinct functional roles of the four heart chambers. Female mice were not investigated owing to their transcriptome dependence on the estrous cycle phase. Out of the quantified 16,886 unigenes, 15.76% on the left side and 16.5% on the right side exhibited differential expression between the atrium and the ventricle, while 5.8% of genes were differently expressed between the two atria and only 1.2% between the two ventricles. The study revealed also chamber differences in gene expression control and coordination. We analyzed ion channels and transporters, and genes within the cardiac muscle contraction, oxidative phosphorylation, glycolysis/gluconeogenesis, calcium and adrenergic signaling pathways. Interestingly, while expression of Ank2 oscillates in phase with all 27 quantified binding partners in the left ventricle, the percentage of in-phase oscillating partners of Ank2 is 15% and 37% in the left and right atria and 74% in the right ventricle. The analysis indicated high interventricular synchrony of the ion channels expressions and the substantially lower synchrony between the two atria and between the atrium and the ventricle from the same side.
从 4 只成年雄性 C57Bl/6j 小鼠的左心房和右心房和心室组织中提取心肌转录组，并用安捷伦微阵列进行分析，以确定导致四个心腔不同功能角色的差异。由于雌性小鼠的转录组依赖于发情周期阶段，因此未对其进行研究。在所量化的 16886 个基因中，左侧有 15.76%，右侧有 16.5%的基因在心房和心室之间表达差异，而两个心房之间有 5.8%的基因表达差异，只有两个心室之间有 1.2%的基因表达差异。该研究还揭示了心脏腔室在基因表达调控和协调方面的差异。我们分析了离子通道和转运蛋白，以及心肌收缩、氧化磷酸化、糖酵解/糖异生、钙和肾上腺素能信号通路中的基因。有趣的是，虽然 Ank2 在左心室中与所有 27 个定量结合伴侣同步振荡，但 Ank2 的同步振荡伴侣百分比在左心房和右心房中分别为 15%和 37%，在右心室中为 74%。该分析表明，离子通道表达具有较高的室间同步性，而两个心房之间以及同侧的心房和心室之间的同步性要低得多。
